Sipos Tamás-Csaba, Attila Kövecsi, Kocsis Lóránd, Bălașa Adrian, Chinezu Rareș, Baróti Beáta Ágota, Pap Zsuzsánna
Department of Anatomy and Embryology, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Târgu Mures, 38 Gheorghe Marinescu Str., 540142 Târgu Mures, Romania.
Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Târgu Mures, 540142 Târgu Mures, Romania.
Int J Mol Sci. 2024 Dec 4;25(23):13043. doi: 10.3390/ijms252313043.
Glioblastoma is considered the most aggressive tumor of the central nervous system. The tumor microenvironment includes several components, such as endothelial cells, immune cells, and extracellular matrix components like matrix metalloproteinase-9 (MMP-9), which facilitates the proliferation of endothelial cells with pro-angiogenic roles. The MRI characteristics of glioblastomas can contribute to determining the prognosis. The aim of this study was to analyze the relationship between tumor angiogenesis in glioblastomas in association with MMP-9 immunoexpression. The results were correlated with the Ki-67 proliferation index, p53 immunoexpression, and the mutational status of and , as well as MRI imaging data. This retrospective study included forty-four patients diagnosed with glioblastoma at the Department of Pathology, Târgu Mureș County Emergency Clinical Hospital. MMP-9 immunoexpression was observed in approximately half of the cases, more frequently in patients over 65 years old. Comparing the imaging data with the immunohistochemical results, we observed that the median tumor volume was higher in glioblastomas with and p53 mutations, wild-type status, negative MMP-9 expression, and high Ki-67 proliferation indexes. The median values of MVD-CD34 and MVD-CD105 were higher in cases with extensive peritumoral edema in the contralateral hemisphere. Additionally, mutations were frequently associated with a more pronounced deviation of the median structures. To statistically validate the associations between MRI and the histopathological features of glioblastomas, further studies with larger cohorts are required.
胶质母细胞瘤被认为是中枢神经系统中最具侵袭性的肿瘤。肿瘤微环境包括几个组成部分，如内皮细胞、免疫细胞以及细胞外基质成分，如基质金属蛋白酶-9（MMP-9），它促进具有促血管生成作用的内皮细胞增殖。胶质母细胞瘤的MRI特征有助于判断预后。本研究的目的是分析胶质母细胞瘤中肿瘤血管生成与MMP-9免疫表达之间的关系。结果与Ki-67增殖指数、p53免疫表达、以及 和 的突变状态以及MRI成像数据相关。这项回顾性研究纳入了44例在特尔古穆列什县急诊临床医院病理科被诊断为胶质母细胞瘤的患者。在大约一半的病例中观察到MMP-9免疫表达，在65岁以上的患者中更常见。将成像数据与免疫组化结果进行比较，我们观察到在具有 和p53突变、 野生型状态、MMP-9阴性表达以及高Ki-67增殖指数的胶质母细胞瘤中，肿瘤体积中位数更高。在对侧半球有广泛瘤周水肿的病例中，MVD-CD34和MVD-CD105的中位数更高。此外， 突变常与中位数结构的更明显偏差相关。为了从统计学上验证MRI与胶质母细胞瘤组织病理学特征之间的关联，需要对更大的队列进行进一步研究。
