Fu Xue, Tang Xun, Zhang Ning, Si Huaijun
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.
College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
Int J Mol Sci. 2024 Dec 8;25(23):13197. doi: 10.3390/ijms252313197.
Protein ubiquitination is an important regulatory mechanism for biological growth and development against environmental influences, and can affect several biological processes, including the growth, development, and stress responses of plants. However, the function of potato-related ubiquitin-conjugating enzymes in abiotic stress tolerance is poorly understood. In this study, a with a UBC conserved structural domain was identified in potato and its function was investigated under osmotic stress and salt stress conditions. The observation of plant phenotypes under stress conditions revealed that overexpressed plants grew better than wild-type plants. In line with the above results, the determination of stress-related physiological indices revealed that the overexpression transgenic plants had better stress tolerance and stronger adaptation to environmental stress, and the transgenic plants were found to tolerate better drought and salt stress by decreasing their malondialdehyde (MDA) content and increasing their superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) contents under stress conditions. Based on these results, StUBC13 has an important regulatory role in the response of plants to abiotic stresses (osmotic stress and salt stress), and overexpression of this gene can improve the tolerance of potatoes to osmotic and salt stresses.
蛋白质泛素化是生物生长发育对抗环境影响的一种重要调控机制，并且会影响多个生物学过程，包括植物的生长、发育和应激反应。然而，马铃薯相关泛素结合酶在非生物胁迫耐受性方面的功能却鲜为人知。在本研究中，在马铃薯中鉴定出一个具有UBC保守结构域的基因，并对其在渗透胁迫和盐胁迫条件下的功能进行了研究。胁迫条件下对植物表型的观察表明，过表达植株比野生型植株生长得更好。与上述结果一致，对胁迫相关生理指标的测定表明，过表达转基因植株具有更好的胁迫耐受性和更强的环境胁迫适应性，并且发现转基因植株通过在胁迫条件下降低丙二醛（MDA）含量、增加超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）含量，从而更好地耐受干旱和盐胁迫。基于这些结果，StUBC13在植物对非生物胁迫（渗透胁迫和盐胁迫）的响应中具有重要的调控作用，该基因的过表达可以提高马铃薯对渗透胁迫和盐胁迫的耐受性。
