Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China.
Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China.
Int J Mol Sci. 2024 Nov 19;25(22):12423. doi: 10.3390/ijms252212423.
GATA factors are evolutionarily conserved transcription regulators that are implicated in the regulation of physiological changes under abiotic stress. Unfortunately, there are few studies investigating the potential role of genes in potato plants responding to salt and osmotic stresses. The physicochemical properties, chromosomal distribution, gene duplication, evolutionary relationships and classification, conserved motifs, gene structure, interspecific collinearity relationship, and cis-regulatory elements were analyzed. Potato plants were treated with NaCl and PEG to induce salinity and osmotic stress responses. qRT-PCR was carried out to characterize the expression pattern of StGATA family genes in potato plants subjected to salinity and osmotic stress. loss-of-function and gain-of-function plants were established. Morphological phenotypes and growth were indicated. Photosynthetic gas exchange was suggested by the net photosynthetic rate, transpiration rate, and stomatal conductance. Physiological indicators and the corresponding genes were indicated by enzyme activity and mRNA expression of genes encoding CAT, SOD, POD, and P5CS, and contents of HO, MDA, and proline. The expression patterns of StGATA family genes were altered in response to salinity and osmotic stress. StGATA12 protein is located in the nucleus. is involved in the regulation of potato plant growth in response to salinity and osmotic stress. Overexpression of promoted photosynthesis, transpiration, and stomatal conductance under salinity and osmotic stress. overexpression induced biochemical responses of potato plants to salinity and osmotic stress by regulating the levels of HO, MDA, and proline and the activity of CAT, SOD, and POD. overexpression induced the up-regulation of , , , and against salinity and osmotic stress. could reinforce the ability of potato plants to resist salinity and osmosis-induced damages, which may provide an effective strategy to engineer potato plants for better adaptability to adverse salinity and osmotic conditions.
GATA 因子是进化上保守的转录调控因子，它们参与调节非生物胁迫下的生理变化。不幸的是，目前很少有研究调查基因在马铃薯植物对盐和渗透胁迫的响应中的潜在作用。对其理化性质、染色体分布、基因复制、进化关系和分类、保守基序、基因结构、种间共线性关系和顺式调控元件进行了分析。用 NaCl 和 PEG 处理马铃薯植株，以诱导盐度和渗透胁迫反应。进行 qRT-PCR 以表征马铃薯植株中 StGATA 家族基因在盐度和渗透胁迫下的表达模式。建立了 基因的功能丧失和功能获得植物。表型和生长形态指示。净光合速率、蒸腾速率和气孔导度表明光合作用气体交换。通过编码 CAT、SOD、POD 和 P5CS 的基因的酶活性和 mRNA 表达以及 HO、MDA 和脯氨酸的含量指示生理指标和相应基因。StGATA 家族基因的表达模式在盐度和渗透胁迫下发生改变。StGATA12 蛋白位于细胞核内。参与调节马铃薯植物对盐度和渗透胁迫的生长。过表达 在盐度和渗透胁迫下促进光合作用、蒸腾作用和气孔导度。过表达通过调节 HO、MDA 和脯氨酸的水平以及 CAT、SOD 和 POD 的活性，诱导马铃薯植株对盐度和渗透胁迫的生化反应。过表达诱导 对盐度和渗透胁迫的上调。过表达可增强马铃薯植株抵抗盐度和渗透胁迫诱导损伤的能力，这可能为工程马铃薯植物提供一种有效策略，以提高其对不利盐度和渗透条件的适应性。
