Zhang Chuwei, Zhang Xiaoyi, Li Fei, Li Bo, Zhang Mengnan, Li Wanqian, Zhuge Pan, Yao Jingye, Zhang Yi, Chen Shixuan, Fang Yongjin, Cai Chao
Department of Burn and Plastic Surgery, Department of Wound Repair Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, China.
Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of the Chinese Academy of Sciences, Wenzhou, Zhejiang, 325000, China.
Adv Sci (Weinh). 2025 Feb;12(6):e2411575. doi: 10.1002/advs.202411575. Epub 2024 Dec 16.
Effective regulation and reconstruction of the microenvironment are critical for the regeneration of chronic wounds. Diabetic wounds, in particular, pose a significant clinical challenge due to increased oxidative stress and dysfunctional healing processes. In this study, a novel therapeutic strategy is developed using 3D copper-magnesium bimetallic antioxidant nano-enzymes (Cu/Mg-MOF) to mitigate reactive oxygen species (ROS) and restore redox balance through electron transfer. To optimize delivery, a thermo-sensitive hydrogel composed of chitosan (CS) and ε-polylysine (PL) is designed, serving as an efficient carrier for the nano-enzymes. This Cu/Mg-MOF@CS/PL hydrogel exhibits excellent physical properties, including injectability, softness, and biocompatibility, making it ideal for application in diabetic wounds. In a diabetic wound model, treatment with Cu/Mg-MOF@CS/PL hydrogel significantly accelerated wound healing, with a closure rate of 90.6% by day 14, compared to just 55.4% in the untreated group. The hydrogel effectively promoted key aspects of wound healing, such as collagen deposition, re-epithelialization, angiogenesis, and immunomodulation. These findings underscore the potential of the Cu/Mg-MOF@CS/PL hydrogel as a promising therapeutic system for enhancing the healing of diabetic wounds.
对微环境进行有效的调节和重建对于慢性伤口的再生至关重要。尤其是糖尿病伤口，由于氧化应激增加和愈合过程功能失调，构成了重大的临床挑战。在本研究中，开发了一种新的治疗策略，使用三维铜镁双金属抗氧化纳米酶（Cu/Mg-MOF）来减轻活性氧（ROS）并通过电子转移恢复氧化还原平衡。为了优化递送，设计了一种由壳聚糖（CS）和ε-聚赖氨酸（PL）组成的热敏水凝胶，作为纳米酶的有效载体。这种Cu/Mg-MOF@CS/PL水凝胶具有优异的物理性质，包括可注射性、柔软性和生物相容性，使其非常适合应用于糖尿病伤口。在糖尿病伤口模型中，用Cu/Mg-MOF@CS/PL水凝胶治疗可显著加速伤口愈合，到第14天时闭合率为90.6%，而未治疗组仅为55.4%。该水凝胶有效地促进了伤口愈合的关键方面，如胶原蛋白沉积、再上皮化、血管生成和免疫调节。这些发现强调了Cu/Mg-MOF@CS/PL水凝胶作为一种有前景的治疗系统用于促进糖尿病伤口愈合的潜力。
