Hu Xulin, Chen Jiao, Yang Shuhao, Zhang Zhen, Wu Haoming, He Jian, Qin Leilei, Cao Jianfei, Xiong Chengdong, Li Kainan, Liu Xian, Qian Zhiyong
Clinical Medical College and Affiliated Hospital of Chengdu University, Chengdu University, Chengdu, Sichuan, 610081, China.
Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Small. 2024 Oct;20(40):e2403681. doi: 10.1002/smll.202403681. Epub 2024 May 28.
Infected bone defects are one of the most challenging problems in the treatment of bone defects due to the high antibiotic failure rate and the lack of ideal bone grafts. In this paper, inspired by clinical bone cement filling treatment, α-c phosphate (α-TCP) with self-curing properties is composited with β-tricalcium phosphate (β-TCP) and constructed a bionic cancellous bone scaffolding system α/β-tricalcium phosphate (α/β-TCP) by low-temperature 3D printing, and gelatin is preserved inside the scaffolds as an organic phase, and later loaded with a metal-polyphenol network structure of tea polyphenol-magnesium (TP-Mg) nanoparticles. The scaffolds mimic the structure and components of cancellous bone with high mechanical strength (>100 MPa) based on α-TCP self-curing properties through low-temperature 3D printing. Meanwhile, the scaffolds loaded with TP-Mg exhibit significant inhibition of Staphylococcus aureus (S.aureus) and promote the transition of macrophages from M1 pro-inflammatory to M2 anti-inflammatory phenotype. In addition, the composite scaffold also exhibits excellent bone-enhancing effects based on the synergistic effect of Mg and Ca. In this study, a multifunctional ceramic scaffold (α/β-TCP@TP-Mg) that integrates anti-inflammatory, antibacterial, and osteoinduction is constructed, which promotes late bone regenerative healing while modulating the early microenvironment of infected bone defects, has a promising application in the treatment of infected bone defects.
由于抗生素失败率高且缺乏理想的骨移植材料，感染性骨缺损是骨缺损治疗中最具挑战性的问题之一。本文受临床骨水泥填充治疗的启发，将具有自固化性能的α-磷酸钙（α-TCP）与β-磷酸三钙（β-TCP）复合，并通过低温3D打印构建了一种仿生松质骨支架系统α/β-磷酸三钙（α/β-TCP），明胶作为有机相保留在支架内部，随后负载茶多酚-镁（TP-Mg）纳米颗粒的金属-多酚网络结构。基于α-TCP的自固化性能，通过低温3D打印，该支架模仿了松质骨的结构和成分，具有较高的机械强度（>100 MPa）。同时，负载TP-Mg的支架对金黄色葡萄球菌（S.aureus）具有显著的抑制作用，并促进巨噬细胞从M1促炎表型向M2抗炎表型的转变。此外，基于Mg和Ca的协同作用，复合支架还表现出优异的骨增强效果。在本研究中，构建了一种集成抗炎、抗菌和骨诱导功能的多功能陶瓷支架（α/β-TCP@TP-Mg），其在调节感染性骨缺损早期微环境的同时促进晚期骨再生愈合，在感染性骨缺损治疗中具有广阔的应用前景。
