Nakagawa Kaoru, Iida Tetsuo, Kobayashi Manami, Shimma Shuichi
Solutions COE, Analytical & Measuring Instruments Division, Shimadzu Corporation, Kyoto, 1 Nishinokyo Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan.
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Mass Spectrom (Tokyo). 2024;13(1):A0163. doi: 10.5702/massspectrometry.A0163. Epub 2024 Dec 10.
Plant alkaloids are used in a variety of medicines, such as anti-cancer drugs and analgesics. Among these plant alkaloids, galanthamine is an Amaryllidaceae type alkaloid with acetylcholinesterase inhibition activity used in the treatment of neurological diseases such as Alzheimer's disease. Although the chemical synthesis of galanthamine has been successfully achieved, is the main source of its production. Research shows that the galanthamine content varies not only with the type of daffodil but also with the stage of development and the part of the plant. Pharmaceutical companies seek plant species with higher galanthamine content to increase pharmaceutical availability. In this study, we were able to rapidly confirm the presence of alkaloids, such as galanthamine, lycorine, and tazettine in the sample using the probe electrospray ionization coupled with quadrupole time-of-flight type mass spectrometry. After confirmation of the components, we then visualized the distribution of the alkaloids by mass spectrometry imaging using the atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometer. In our method, we can provide qualitative data and visualized data immediately.
植物生物碱被用于多种药物中，如抗癌药物和镇痛药。在这些植物生物碱中，加兰他敏是一种具有乙酰胆碱酯酶抑制活性的石蒜科生物碱，用于治疗阿尔茨海默病等神经疾病。尽管加兰他敏的化学合成已成功实现，但 是其生产的主要来源。研究表明，加兰他敏的含量不仅因水仙种类而异，还因发育阶段和植物部位而异。制药公司寻找加兰他敏含量较高的植物物种以提高药物可得性。在本研究中，我们能够使用探针电喷雾电离结合四极杆飞行时间型质谱快速确认样品中加兰他敏、石蒜碱和水仙环素等生物碱的存在。在确认成分后，我们随后使用大气压基质辅助激光解吸/电离质谱仪通过质谱成像可视化生物碱的分布。在我们的方法中，我们可以立即提供定性数据和可视化数据。
