Afzal Sheryar, Wu Yuan Seng, Manap Aimi Syamaima Abdul, Attiq Ali, Albokhadaim Ibrahim, Appalaraju Velaga, Khalid Ahmed Magzoub, Eseyin Olorunfemi
Department of Biomedical Science, College of Veterinary Medicine, King Faisal University, Hofuf, Saudi Arabia.
Department of Medical Education, School of Medical and Life Sciences, Sunway University, Selangor, Malaysia.
Indian J Pharmacol. 2024 Sep 1;56(5):329-334. doi: 10.4103/ijp.ijp_564_24. Epub 2024 Dec 16.
Sansevieria trifasciata, common name, mother-in-law's tongue, is a member of the Agavaceae family. We undertook this study to evaluate the cytotoxicity of S. trifasciata leaf extract against two cancer cell lines as well as its antibacterial activities against six bacterial strains.
The investigated cell lines include primary colon epithelial (PCE) cells and human colorectal cancer cells; the studied bacterial strains are Staphylococcus aureus, Proteus vulgaris, Bacillus subtilis, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Escherichia coli. Using the agar well-diffusion method, various doses (5, 10, and 20 mg/mL) of plant extracts (ethanol and petroleum ether) were evaluated against each kind of bacterial strain. The minimal inhibitory doses were found using the two-fold serial dilution approach, with a range of 0.156-5 mg/mL.
Comparing extracts of S. trifasciata leaves to tetracycline (0.05 mg/mL), a common antibiotic, revealed a wide range of antibacterial activity. P. vulgaris and S. aureus were the most sensitive bacterial strains to ethanol and petroleum ether extracts, respectively. The MTT test was employed to ascertain the viable cell count of PCE cells and HCT-116. When various ethanol extract concentrations (7.8, 15.63, 31.25, 62.5, 125, 250, 500, and 1000 μg/mL) were tested against the cell lines, HCT-116's IC50, values were lower as compared to PCE. The IC50 values for HCT-116 and PCE cells ranged from 10.0 to 14.07 μg/mL and 92.9-216.9 μg/mL, respectively.
Ethanolic extract of S. trifasciata showed promising antibacterial and anticancer properties.
虎尾兰，俗名岳母舌，是龙舌兰科植物的一种。我们开展本研究以评估虎尾兰叶提取物对两种癌细胞系的细胞毒性及其对六种细菌菌株的抗菌活性。
所研究的细胞系包括原代结肠上皮（PCE）细胞和人结肠癌细胞；所研究的细菌菌株为金黄色葡萄球菌、普通变形杆菌、枯草芽孢杆菌、肺炎克雷伯菌、铜绿假单胞菌和大肠杆菌。采用琼脂扩散法，针对每种细菌菌株评估不同剂量（5、10和20 mg/mL）的植物提取物（乙醇和石油醚提取物）。使用两倍系列稀释法确定最小抑制剂量，范围为0.156 - 5 mg/mL。
将虎尾兰叶提取物与常见抗生素四环素（0.05 mg/mL）进行比较，发现其具有广泛的抗菌活性。普通变形杆菌和金黄色葡萄球菌分别是对乙醇提取物和石油醚提取物最敏感的细菌菌株。采用MTT试验确定PCE细胞和HCT - 116的活细胞数量。当针对细胞系测试不同乙醇提取物浓度（7.8、15.63、31.25、62.5、125、250、500和1000 μg/mL）时，与PCE相比，HCT - 116的IC50值较低。HCT - 116和PCE细胞的IC50值分别为10.0至14.07 μg/mL和92.9 - 216.9 μg/mL。
虎尾兰乙醇提取物显示出有前景的抗菌和抗癌特性。
