Morais Violeta Nunes de, Moreira Luiza de Paula Dias, Gomes Mariana Juste Contin, Grancieri Mariana, Lucio Haira Guedes, Toledo Renata Celi Lopes, Mishima Marcella Duarte Villas, Costa Neuza Maria Brunoro, da Silva Bárbara Pereira, Stampini Duarte Martino Hércia
Department of Nutrition and Health, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Viken, Norway.
J Am Nutr Assoc. 2025 May-Jun;44(4):322-331. doi: 10.1080/27697061.2024.2431271. Epub 2024 Dec 17.
A diet rich in fat and sugar is present in society everyday life, leading to the development of metabolic changes, especially in intestinal microbiota. Chia oil is a source of alpha-linolenic acid, which has antioxidant and anti-glycemic effects. Based on this, we hypothesized that chia oil may promote intestinal health.
The study aims to investigate the effects of chia oil on gut microbiota and intestinal health in rats fed a high-fat and high-fructose diet (HFHF).
The animals were separated into two groups and received the following diets: standard murine diet (AIN-93M) ( = 10) and HFHF ( = 20) to induce metabolic changes (phase I) during eight weeks. After that, the AIN-93M group remained unchanged, while the HFHF group was divided into two groups: HFHF ( = 10) and HFHF with chia oil (HFHF+CO) ( = 10) for ten weeks (phase II, chia oil treatment). We analyzed immunoglobulin A (IgA) levels, cecal pH, short-chain fatty acids (SCFAs), intestinal permeability, intestinal microbiome composition, histomorphometry, and murinometric parameters.
Chia oil consumption increased alpha-linolenic acid intake, IgA levels, propionic acid production, cecum weight, goblet cell number, thickness and depth of intestinal crypts, and the thickness of both circular and longitudinal muscle layers of the colon, and decreased cecal pH. No change was observed in the alpha and beta diversity between the HFHF and HFHF+CO groups. The HFHF+CO diet increased the relative abundance of genera sp., sp., and sp., compared to the AIN-93M group. No difference was observed in the intestinal permeability among the groups.
Chia oil consumption is an alternative for improving the intestinal health of rats fed a HFHF diet.
高脂肪和高糖饮食在社会日常生活中普遍存在，会导致代谢变化，尤其是肠道微生物群的变化。奇亚籽油是α-亚麻酸的来源，具有抗氧化和抗血糖作用。基于此，我们推测奇亚籽油可能促进肠道健康。
本研究旨在探讨奇亚籽油对高脂高糖饮食（HFHF）大鼠肠道微生物群和肠道健康的影响。
将动物分为两组，给予以下饮食：标准鼠粮（AIN-93M）（n = 10）和HFHF（n = 20），持续八周以诱导代谢变化（第一阶段）。之后，AIN-93M组保持不变，而HFHF组分为两组：HFHF（n = 10）和添加奇亚籽油的HFHF组（HFHF+CO）（n = 10），持续十周（第二阶段，奇亚籽油处理）。我们分析了免疫球蛋白A（IgA）水平、盲肠pH值、短链脂肪酸（SCFAs）、肠道通透性、肠道微生物群组成、组织形态计量学和鼠类测量参数。
食用奇亚籽油增加了α-亚麻酸摄入量、IgA水平、丙酸产量、盲肠重量、杯状细胞数量、肠隐窝的厚度和深度以及结肠环形和纵形肌层的厚度，并降低了盲肠pH值。HFHF组和HFHF+CO组之间的α和β多样性没有变化。与AIN-93M组相比，HFHF+CO饮食增加了属、属和属的相对丰度。各组之间的肠道通透性没有差异。
食用奇亚籽油是改善HFHF饮食大鼠肠道健康的一种选择。
