Unfolding bovine serum albumin decorated selenium nanoparticles crosslinking with chitosan: Achieve stabilization of Pickering emulsions gel and enhance resveratrol bioaccessibility.

Resveratrol (Res) is a natural polyphenol exhibiting anti-oxidant and anti-inflammatory activity. However, the applications of Res have been limited due to its low stability and water solubility. To enhance the bioaccessibility of Res, unfolding bovine serum albumin-modified selenium nanoparticles (UBSA@SeNPs) encapsulated within chitosan (CS)-coated Pickering emulsions (CS-UBSA@SeNPs-PE) were used to load Res. The results showed that Res-loaded CS(0.06 %)-UBSA@SeNPs-PE has small droplet size (16.13 μm), high gel properties and excellent antioxidant properties. During the simulated digestion process, CS reduced the release rate of Res from Res-loaded CS(0.06 %)-UBSA@SeNPs-PE (42.27 %) to reach a slow release effect. Importantly, Res could quickly release from CS-UBSA@SeNPs-PE within intestinal fluid or in the presence of chitosanase. In simulated absorption experiments, the intestinal permeability of Res in Res-loaded CS(0.06 %)-UBSA@SeNPs-PE were enhanced by 292.31 % compare to Res-loaded CS(0 %)-UBSA@SeNPs-PE. In pharmacokinetic studies, Res-loaded CS(0.06 %)-UBSA@SeNPs-PE had an area under the curve (AUC) up to 3467.99 ± 127.43 ng*h/mL. Furthermore, CS also improved the mucoadhesive nature of UBSA@SeNPs-PE, resulting in a gut-retention time of Res-loaded CS(0.06 %)-UBSA@SeNPs-PE that reached up to 60 h. In conclusion, CS-UBSA@SeNPs-PE can serve as an effective oral delivery system for improving the bioaccessibility of Res.