Wang Wenpei, Tan Qiangfei, Wang Qiaoli, Wang Jing, Zhang Fanhong, Zheng Xue, Yun Jianmin, Zhang Wenwei, Zhao Fengyun
College of Food Science and Engineering, Functional Dairy Products Engineering Laboratory of Gansu Province, Gansu Agricultural University, Lanzhou 730070, China; Lanzhou Institute of Biological Products Limited Liability Company, Lanzhou 730046, China.
College of Food Science and Engineering, Functional Dairy Products Engineering Laboratory of Gansu Province, Gansu Agricultural University, Lanzhou 730070, China.
Int J Biol Macromol. 2025 Feb;289:138835. doi: 10.1016/j.ijbiomac.2024.138835. Epub 2024 Dec 15.
Degradation of Volvariella volvacea is associated with the accumulation of reactive oxygen species (ROS), and glutathione peroxidase (GPX) is one of the key antioxidant enzyme. The purpose of this research is to uncover the importance of the gpx gene in the degradation and revitalization of V. volvacea. In this study, a gpx-silenced strain of the primordial strain T0 and an overexpression strain of the degraded strain T19 of V. volvacea were constructed, and their antioxidant properties, matrix degradation ability, and production traits were determined. The results showed that the expression level of gpx altered the homeostasis of the V. volvacea redox system and affected the substrate degradation ability of V. volvacea, which altered the physiological traits of the V. volvacea mycelium. Most importantly, the primordial strain T0 was unable to produce fruiting bodies due to the silence of the gpx gene. On the other hand, the gpx gene overexpression promoted the regrowth of fruiting bodies in degenerated strains of V. volvacea T19. This study provides a new biotechnological strategy to control the degeneration of V. volvacea and other edible fungi.
草菇的降解与活性氧（ROS）的积累有关，而谷胱甘肽过氧化物酶（GPX）是关键的抗氧化酶之一。本研究的目的是揭示gpx基因在草菇降解和复壮中的重要性。在本研究中，构建了草菇原始菌株T0的gpx基因沉默菌株和降解菌株T19的过表达菌株，并测定了它们的抗氧化特性、基质降解能力和生产性状。结果表明，gpx基因的表达水平改变了草菇氧化还原系统的稳态，影响了草菇的底物降解能力，进而改变了草菇菌丝体的生理特性。最重要的是，由于gpx基因沉默，原始菌株T0无法产生子实体。另一方面，gpx基因过表达促进了草菇退化菌株T19中子实体的再生。本研究为控制草菇和其他食用菌的退化提供了一种新的生物技术策略。
