Wang Qiaoli, Zhu Jianing, Wang Yonghui, Yun Jianmin, Zhang Yubin, Zhao Fengyun
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
Kangle County Special Agricultural Development Center, Linxia 731599, China.
J Fungi (Basel). 2024 Aug 1;10(8):540. doi: 10.3390/jof10080540.
Serine is a functional amino acid that effectively regulates the physiological functions of an organism. This study investigates the effects of adding exogenous serine to a culture medium to explore a feasible method for the rejuvenation of degenerated strains. The tissue isolation subcultured strains T6, T12, and T19 of were used as test strains, and the commercially cultivated strain V844 (T0) was used as a control. The results revealed that the addition of serine had no significant effect on non-degenerated strains T0 and T6, but could effectively restore the production characteristics of degenerated strains T12 and T19. Serine increased the biological efficiency of T12 and even helped the severely degenerated T19 to regrow its fruiting body. Moreover, exogenous serine up-regulated the expression of some antioxidant enzyme genes, improved antioxidase activity, reduced the accumulation of reactive oxygen species (ROS), lowered malondialdehyde (MDA) content, and restored mitochondrial membrane potential (MMP) and mitochondrial morphology. Meanwhile, serine treatment increased lignocellulase and mycelial energy levels. These findings form a theoretical basis and technical support for the rejuvenation of degenerated strains and other edible fungi.
丝氨酸是一种功能性氨基酸，可有效调节生物体的生理功能。本研究通过在培养基中添加外源丝氨酸，探究退化菌株复壮的可行方法。以组织分离继代培养的菌株T6、T12和T19为试验菌株，以商业栽培菌株V844（T0）作为对照。结果表明，添加丝氨酸对未退化菌株T0和T6无显著影响，但能有效恢复退化菌株T12和T19的生产特性。丝氨酸提高了T12的生物学效率，甚至帮助严重退化的T19重新长出子实体。此外，外源丝氨酸上调了一些抗氧化酶基因的表达，提高了抗氧化酶活性，减少了活性氧（ROS）的积累，降低了丙二醛（MDA）含量，恢复了线粒体膜电位（MMP）和线粒体形态。同时，丝氨酸处理提高了木质纤维素酶和菌丝体能量水平。这些研究结果为退化菌株及其他食用菌的复壮提供了理论依据和技术支持。
