Gregory Eli S, Xu YiFeng Y J, Lee Tai-Ting, Joiner Mei-Ling A, Kamikouchi Azusa, Su Matthew P, Eberl Daniel F
Department of Biology, University of Iowa, Iowa City, Iowa 52242.
Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
eNeuro. 2025 Jan 9;12(1). doi: 10.1523/ENEURO.0083-24.2024. Print 2025 Jan.
The full complement of ion channels which influence insect auditory mechanotransduction and the mechanisms by which their influence is exerted remain unclear. (K4), a family member encoding voltage-gated potassium channels in , has been shown to localize to dendrites in some neuron types, suggesting the potential role of in hearing, including mechanotransduction. A GFP trap was used to visualize the localization of the channel in Johnston's organ neurons responsible for hearing in the antenna. protein was localized strongly to the cell body and inner dendritic segment of sensory neurons. It was also detectable in the sensory cilium, suggesting its involvement not only in general auditory function but specifically in mechanotransduction. Electrophysiological recordings to assess neural responses to auditory stimuli in mutant flies revealed significant decreases in auditory responses. Laser Doppler vibrometer recordings indicated abnormal antennal free fluctuation frequencies in mutant lines, indicating an effect on active antennal tuning, and thus active transduction mechanisms. This suggests that participates in coordinating energy-dependent antennal movements in that are essential for tuning the antenna to courtship song frequencies.
影响昆虫听觉机械转导的离子通道的完整组成及其发挥作用的机制仍不清楚。（K4）是一种在[具体物种]中编码电压门控钾通道的家族成员，已被证明在某些神经元类型中定位于树突，这表明（K4）在[具体物种]听觉中，包括机械转导方面具有潜在作用。使用绿色荧光蛋白（GFP）陷阱来观察（K4）通道在触角中负责听觉的约翰斯顿氏器神经元中的定位。（K4）蛋白强烈定位于感觉神经元的细胞体和树突内段。在感觉纤毛中也可检测到它，这表明它不仅参与一般听觉功能，而且特别参与机械转导。评估突变型[具体物种]果蝇对听觉刺激的神经反应的电生理记录显示听觉反应显著降低。激光多普勒振动计记录表明突变系中触角自由波动频率异常，这表明对主动触角调谐以及因此对主动转导机制有影响。这表明（K4）参与协调[具体物种]中依赖能量的触角运动，这些运动对于将触角调谐到求偶歌曲频率至关重要。
