Oluranti Olayinka Oluyemi, Ogundeji Babatunde Ayodeji, Orisajo Samuel Bukola
Microbiology Programme, Bowen University, Iwo, Osun State, Nigeria.
Plant Pathology Section, Cocoa Research Institute of Nigeria, Ibadan, Oyo State, Nigeria.
Discov Nano. 2024 Dec 18;19(1):207. doi: 10.1186/s11671-024-04113-6.
Silver nanoparticle solutions (AgNPs) of some mushrooms: Pleurotus ostreatus, Agaricus bisporus and Agaricus campestris were prepared and characterized using Transmission Electron Microscopy (TEM), Fourier-Transform Infrared (FTIR) spectroscopy, X-Ray Diffraction (XRD) analysis and Energy Dispersive X-ray (EDX) spectroscopy. Each of the myco-sythesized AgNPs was plated against strains of Aspergillus flavus and A. ochraceous, at 5, 10 and 15% concentrations. Colour change from light yellow to orange, yellowish-brown, and reddish brown was observed after overnight incubation (at 28 °C), in the P. ostreatus, A. bisporus, and A. campestris synthesized AgNPs respectively. TEM analysis showed a spherical shape with an average size of 15.25 to 45.85 nm, 9.22 to 52.60 nm and 10.24 to 17.66 nm in P. ostreatus, A. campestris and A. bisporus AgNPs respectively. EDX spectrum showed absorption peaks of silver in the ranges of 0.8-1.4 keV, 6.2-6.6 keV, and 0.8-1.2 keV, and XRD analysis confirmed the crystalline structure of the biosynthesized AgNPs, while FTIR results revealed O-H, N-H, C=O, and C=N as the prominent functional groups. Mycelial inhibitions against A. flavus strains D28AF and D42AF ranged between 43.86-52.73% and 33.83-57.07% respectively, and were not significantly different (P ≤ 0.05) from the standard (copper sulphate). Inhibitions produced against A. ochraceous strains AOD40 and AOD45 ranged between 34.64-52.36% and 37.43-53.56% respectively and also showed similar trend in relation to the standard. This study showed that the myco-synthesized AgNPs were effective against A. flavus and A. ochraceous infecting cocoa beans at storage. They however need to be further improved for future use in the control of cocoa beans pathogens.
制备了平菇、双孢蘑菇和野蘑菇的银纳米颗粒溶液（AgNPs），并使用透射电子显微镜（TEM）、傅里叶变换红外（FTIR）光谱、X射线衍射（XRD）分析和能量色散X射线（EDX）光谱对其进行了表征。将每种真菌合成的AgNPs以5%、10%和15%的浓度接种到黄曲霉和赭曲霉菌株上。过夜培养（28℃）后，分别在平菇、双孢蘑菇和野蘑菇合成的AgNPs中观察到颜色从浅黄色变为橙色、黄棕色和红棕色。TEM分析显示，平菇、野蘑菇和双孢蘑菇AgNPs的形状均为球形，平均尺寸分别为15.25至45.85纳米、9.22至52.60纳米和10.24至17.66纳米。EDX光谱显示银的吸收峰在0.8 - 1.4keV、6.2 - 6.6keV和0.8 - 1.2keV范围内，XRD分析证实了生物合成AgNPs的晶体结构，而FTIR结果显示O - H、N - H、C = O和C = N为主要官能团。对黄曲霉菌株D28AF和D42AF的菌丝体抑制率分别在43.86 - 52.73%和33.83 - 57.07%之间，与标准品（硫酸铜）无显著差异（P≤0.05）。对赭曲霉菌株AOD40和AOD45产生的抑制率分别在34.64 - 52.36%和37.43 - 53.56%之间，与标准品也呈现相似趋势。本研究表明，真菌合成的AgNPs对储存期感染可可豆的黄曲霉和赭曲霉有效。然而，它们在未来用于控制可可豆病原体方面还需要进一步改进。
