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两种 植物来源的生物成因银纳米粒子及其抗菌活性。

Biogenic Silver Nanoparticles from Two Varieties of and Their Antibacterial Activity.

机构信息

Biochemistry Department, College of Science, King Saud University, Riyadh 11495, Saudi Arabia.

出版信息

Molecules. 2022 Nov 7;27(21):7656. doi: 10.3390/molecules27217656.

Abstract

, the most widely cultivated mushroom, is safe to eat and enriched with protein and secondary metabolites. We prepared silver nanoparticles (AgNPs) from two varieties of and tested their antibacterial activity The synthesized AgNPs were initially confirmed by UV-Vis spectroscopy peaks at 420 and 430 nm for white and brown mushrooms AgNPs, respectively. AgNPs were further characterized by zeta sizer, transmission electronic microscopy (TEM), Fourier transform infrared (FTIR), and energy-dispersive X-ray spectroscopy (EDX) prior to antibacterial activity by the well diffusion method against six bacterial strains which include , , , , , and TEM results revealed a spherical shape with an average diameter of about 11 nm in the white mushroom extract and 5 nm in the brown mushroom extract. The presence of elemental silver in the prepared AgNPs was confirmed by EDS. The IR spectrum of the extract confirmed the presence of phenols, flavonoids, carboxylic, or amide groups which aided in the reduction and capping of synthesized AgNPs. The AgNPs from both extracts showed almost the same results; however, nanoparticles prepared from brown mushrooms were smaller in size with strong antibacterial activity.

摘要

,最广泛栽培的蘑菇,安全可食用,富含蛋白质和次生代谢物。我们从两种蘑菇中制备了银纳米粒子 (AgNPs),并测试了它们的抗菌活性。合成的 AgNPs 最初通过 UV-Vis 光谱在 420nm 和 430nm 处的峰来确认,分别为白色和棕色蘑菇 AgNPs。在进行抗菌活性测试之前,通过使用 well 扩散法对六种细菌菌株(包括 、 、 、 、 和 )进行抗菌活性测试之前,AgNPs 通过zeta 粒径仪、透射电子显微镜 (TEM)、傅里叶变换红外 (FTIR) 和能量色散 X 射线光谱 (EDX) 进行了进一步的表征。TEM 结果显示,白色蘑菇提取物中的银纳米粒子呈球形,平均直径约为 11nm,棕色蘑菇提取物中的银纳米粒子呈球形,平均直径约为 5nm。通过 EDS 证实了制备的 AgNPs 中存在元素银。提取物的 IR 光谱证实了酚类、类黄酮、羧酸或酰胺基团的存在,这些基团有助于还原和包覆合成的 AgNPs。两种提取物制备的 AgNPs 几乎具有相同的结果;然而,从小型化和强抗菌活性方面来说,由棕色蘑菇制备的纳米颗粒更小。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2b9/9654042/a1aa5ebc1a86/molecules-27-07656-g001.jpg

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