Abo El-Enin Hadel A, Ali Isra H, Naguib Ibrahim A, Tolba Nahla Sameh, Abdel-Bar Hend Mohamed
Department of Pharmaceutics, Egyptian Drug Authority, Giza, P.O. Box 12511, Egypt.
Department of Pharmaceutics, Faculty of Pharmacy, University of Sadat City, P.O. Box 32897, Menoufia, Egypt; Nanomedicine Laboratory, Faculty of Pharmacy, University of Sadat City, P.O. Box 32897, Sadat City, Egypt.
Int J Biol Macromol. 2025 Apr;298:140371. doi: 10.1016/j.ijbiomac.2025.140371. Epub 2025 Jan 27.
Silver sulfadiazine (SSD) is a widely used antibacterial agent for burn wound treatment owing to its capability in re-epithelialization and wound healing. However, due to its low solubility, the need for an effective drug delivery system is mandatory. This study aimed to optimize SSD nanostructured lipid-based carriers (NLCs), incorporated in a collagen sponge form as an innovative topical dosage form for effective burn wound treatment. SSD-NLCs were prepared by applying Box-Behnken design and characterized in terms of particle size, zeta potential, and entrapment efficiency (EE). The optimized SSD-NLCs formula was selected and incorporated into a cross-linked collagen sponge and lyophilized for 24 h. The SSD-NLCs sponge morphological structure, porosity, swelling ratio, in vitro drug release profile and antibacterial activity were assessed. Furthermore, investigating the competitive inhibitory efficiency of SSD against para-aminobenzoic acid (PABA), the native ligand for the dihydropteroate synthetase enzyme based on the calculated binding free energy using the CB-Dock docking server was evaluated. Additionally, the wound healing activity and histopathological studies were evaluated on a second-degree burn wounds in a rat model. The optimized SSD-NLCs were spherical, possessing size of 115.69 ± 3.25 nm, EE% of 89.69 ± 1.36 % and a porosity of 71.22 %. Furthermore, the SSD-NLCs sponge showed an enhanced swelling ratio and improved antimicrobial activity compared to SSD. Finally, in vivo studies in rats showed that SSD-NLCs sponge are effective wound healing formulation owing to their ability to improve the quality of tissue regeneration without scars formation. Results showed that SSD-NLCs sponge can enhance the SSD efficacy in treatment of burn wounds. Further toxicological studies are still needed before clinical application.
磺胺嘧啶银（SSD）因其具有促进再上皮化和伤口愈合的能力，是一种广泛用于烧伤创面治疗的抗菌剂。然而，由于其溶解度低，必须要有有效的药物递送系统。本研究旨在优化以胶原海绵形式掺入的SSD纳米结构脂质载体（NLCs），作为一种创新的局部剂型用于有效的烧伤创面治疗。通过应用Box-Behnken设计制备SSD-NLCs，并对其粒径、zeta电位和包封率（EE）进行表征。选择优化后的SSD-NLCs配方，将其掺入交联胶原海绵中并冻干24小时。评估了SSD-NLCs海绵的形态结构、孔隙率、溶胀率、体外药物释放曲线和抗菌活性。此外，基于使用CB-Dock对接服务器计算的结合自由能，评估了SSD对二氢蝶酸合酶的天然配体对氨基苯甲酸（PABA）的竞争抑制效率。此外，在大鼠模型的二度烧伤创面上评估了伤口愈合活性和组织病理学研究。优化后的SSD-NLCs呈球形，粒径为115.69±3.25nm，EE%为89.69±1.36%，孔隙率为71.22%。此外，与SSD相比，SSD-NLCs海绵显示出更高的溶胀率和更好的抗菌活性。最后，在大鼠体内的研究表明，SSD-NLCs海绵是有效的伤口愈合制剂，因为它们能够提高组织再生质量而不形成疤痕。结果表明，SSD-NLCs海绵可以提高SSD治疗烧伤创面的疗效。在临床应用之前仍需要进一步的毒理学研究。
