Suraporn Siripuk, Suthikhum Vallaya, Terenius Olle
Department of Biology, Faculty of Science, Mahasarakham University, Kantarawichai District, Mahasarakham, 44150, Thailand.
Center of Excellence for Mulberry and Silk, Mahasarakham University, Kantharawichai District, Mahasarakham, 44150, Thailand.
BMC Res Notes. 2024 Dec 17;17(1):359. doi: 10.1186/s13104-024-07019-9.
Bombyx mori nucleopolyhedrovirus (BmNPV) causes grasserie with severe effects in Thai strains of the silkworm Bombyx mori. The aim of this study was to investigate the effect of probiotic supplementation on the survival of silkworm larvae challenged with BmNPV.
Silkworm larvae of the Thai polyvoltine strain Samrong was supplemented with commercial probiotic bacteria, Lactobacillus acidophilus, on the second day of the 2nd, 3rd, 4th, and 5th instar. When challenged with BmNPV on the second day of the 4th instar, the survival ratio was 92% for larvae supplemented with L. acidophilus as compared to 56% for larvae without L. acidophilus supplementation. For the larvae that survived until pupation, we determined the growth characters cocooning ratio, larval weight, and pupation ratio, and the economic characters cocoon shell weight and cocoon weight. Growth characters were significantly lower in larvae infected with BmNPV as compared to infected larvae receiving probiotics.
家蚕核型多角体病毒（BmNPV）会引发脓病，对泰国品种的家蚕有严重影响。本研究的目的是调查补充益生菌对受BmNPV攻击的家蚕幼虫存活情况的影响。
在泰国多化性品种Samrong的家蚕幼虫2龄、3龄、4龄和5龄的第二天补充商业益生菌嗜酸乳杆菌。在4龄第二天用BmNPV攻击时，补充嗜酸乳杆菌的幼虫存活率为92%，而未补充嗜酸乳杆菌的幼虫存活率为56%。对于存活至化蛹的幼虫，我们测定了生长特性（结茧率、幼虫体重和化蛹率）以及经济特性（茧壳重量和茧重）。与接受益生菌的受感染幼虫相比，感染BmNPV的幼虫的生长特性显著更低。
