Nishida Satoshi, Ishii Masaki, Nishiyama Yayoi, Abe Shigeru, Ono Yasuo, Sekimizu Kazuhisa
Genome Pharmaceuticals Institute Co. Ltd.Tokyo, Japan; Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of TokyoTokyo, Japan; Department of Microbiology and Immunology, Teikyo University School of MedicineTokyo, Japan.
Genome Pharmaceuticals Institute Co. Ltd. Tokyo, Japan.
Front Microbiol. 2017 Mar 20;8:436. doi: 10.3389/fmicb.2017.00436. eCollection 2017.
Lactic acid bacteria (LAB) have high immune system-stimulating activity and are considered beneficial for human health as probiotics in the gut. The innate immune system is highly conserved between mammals and insects. Microbe-associated molecular patterns (e.g., peptidoglycan and β-glucan) induce cytokine maturation, which, in silkworm larvae, leads to muscle contraction. The purpose of this study is to find a novel probiotic by using silkworm muscle contraction assay. In the present study, we isolated LAB derived from rice bran pickles. We selected highly active LAB to activate the innate immune system of the silkworm, which was assayed based on silkworm muscle contraction. Of various LAB, 11-1 strongly stimulated innate immunity in the silkworm, leading to stronger silkworm contraction than a dairy-based LAB. Silkworms fed a diet containing 11-1 exhibited tolerance against the pathogenicity of . These findings suggest that 11-1 could be a useful probiotic for activating innate immunity.
乳酸菌（LAB）具有高度的免疫系统刺激活性，作为肠道中的益生菌被认为对人类健康有益。哺乳动物和昆虫的先天免疫系统高度保守。微生物相关分子模式（如肽聚糖和β-葡聚糖）可诱导细胞因子成熟，这在蚕幼虫中会导致肌肉收缩。本研究的目的是通过使用家蚕肌肉收缩试验来寻找一种新型益生菌。在本研究中，我们从米糠泡菜中分离出乳酸菌。我们选择了高活性乳酸菌来激活家蚕的先天免疫系统，该系统基于家蚕肌肉收缩进行测定。在各种乳酸菌中，11-1强烈刺激家蚕的先天免疫，导致家蚕收缩比基于乳制品的乳酸菌更强。喂食含有11-1的饲料的家蚕表现出对……致病性的耐受性。这些发现表明11-1可能是一种激活先天免疫的有用益生菌。
