Wersebe Matthew J, Günther Torsten, Morton Philip K, Weider Lawrence J, Frisch Dagmar
St. Croix Watershed Research Station Science Museum of Minnesota Marine-on-St. Croix Minnesota USA.
Program in Ecology and Evolutionary Biology, School of Biological Sciences University of Oklahoma Norman Oklahoma USA.
Ecol Evol. 2024 Dec 17;14(12):e70434. doi: 10.1002/ece3.70434. eCollection 2024 Dec.
Most studies of local adaptation substitute the correlation between spatial distance and environmental heterogeneity for the temporal dynamics over which local adaptation evolves. The availability of detailed ecological and genomic information from lake sediments provides an opportunity to study local adaptation with unparalleled clarity from the temporal perspective. Inference can be further enhanced by including multiple lakes along ecological axes to further isolate the effects of ecological change in driving local adaptation. Lakes throughout the world face the impact of numerous anthropogenically induced environmental changes. Top among these is the eutrophication of freshwaters from agriculture, development, and land-use change. Here, we use the genetic information recorded in lake sediments of two lakes experiencing contrasting histories of land-use change to study the evolution of local adaptation in the lakes' populations. Utilizing nextRAD derived single-nucleotide polymorphisms (SNPs) in resurrected clonal lineages, we studied the evolutionary trajectories of in both lakes. Using gene-environment correlations and tests for selection, we found SNPs that appear to be under selection in both lakes. Specifically, we found more outlier SNPs in the highly impacted lake using -based tests for selection. Conversely, gene-environment tests revealed the reverse pattern. We discuss numerous facets of experimental design that must be considered when using resurrection ecology to study local adaptation and critically evaluate how they may have impacted the of this investigation.
大多数关于局部适应性的研究用空间距离与环境异质性之间的相关性来替代局部适应性进化所经历的时间动态。湖泊沉积物中详细的生态和基因组信息的可得性提供了一个从时间角度以前所未有的清晰度研究局部适应性的机会。通过纳入沿生态轴的多个湖泊以进一步分离生态变化在驱动局部适应性方面的影响，推理可以得到进一步加强。世界各地的湖泊都面临着众多人为引起的环境变化的影响。其中最主要的是农业、发展和土地利用变化导致的淡水富营养化。在这里，我们利用记录在经历了不同土地利用变化历史的两个湖泊的沉积物中的遗传信息，来研究湖泊种群中局部适应性的进化。利用在复活的克隆谱系中通过nextRAD获得的单核苷酸多态性（SNP），我们研究了两个湖泊中[具体研究对象未给出]的进化轨迹。通过基因-环境相关性和选择检验，我们发现了在两个湖泊中似乎都受到选择的SNP。具体来说，我们在受影响严重的湖泊中使用基于[具体检验方法未给出]的选择检验发现了更多的异常SNP。相反，基因-环境检验揭示了相反的模式。我们讨论了在使用复活生态学研究局部适应性时必须考虑的实验设计的多个方面，并批判性地评估了它们可能如何影响了本研究的[具体研究结果未明确]。
