Klvanova Eva, Videnska Petra, Barton Vojtech, Bohm Jan, Splichalova Petra, Koksova Viktorie, Urik Milan, Lanickova Barbara, Prokes Roman, Budinska Eva, Klanova Jana, Borilova Linhartova Petra
RECETOX, Faculty of Science, Masaryk University, Brno, Czechia.
Department of Pediatric Otorhinolaryngology, University Hospital Brno, Brno, Czechia.
Front Cell Infect Microbiol. 2024 Dec 3;14:1484100. doi: 10.3389/fcimb.2024.1484100. eCollection 2024.
The antibiotic resistance genes (ARGs) limit the susceptibility of bacteria to antimicrobials, representing a problem of high importance. Current research on the presence of ARGs in microorganisms focuses mainly on humans, livestock, hospitals, or wastewater. However, the spectrum of ARGs in the dust resistome in workplaces and households has gone relatively unexplored. This pilot study aimed to analyze resistome in indoor dust samples from participants' workplaces (a pediatric hospital, a maternity hospital, and a research center) and households and compare two different approaches to the ARGs analysis; high-throughput quantitative PCR (HT-qPCR) and whole metagenome shotgun sequencing (WMGS). In total, 143 ARGs were detected using HT-qPCR, with ARGs associated with the macrolides, lincosamides, and streptogramin B (MLSB) phenotype being the most abundant, followed by MDR (multi-drug resistance) genes, and genes conferring resistance to aminoglycosides. A higher overall relative quantity of ARGs was observed in indoor dust samples from workplaces than from households, with the pediatric hospital being associated with the highest relative quantity of ARGs. WMGS analysis revealed 36 ARGs, of which five were detected by both HT-qPCR and WMGS techniques. Accordingly, the efficacy of the WMGS approach to detect ARGs was lower than that of HT-qPCR. In summary, our pilot data revealed that indoor dust in buildings where people spend most of their time (workplaces, households) can be a significant source of antimicrobial-resistant microorganisms, which may potentially pose a health risk to both humans and animals.
抗生素抗性基因（ARGs）会限制细菌对抗菌药物的敏感性，这是一个极为重要的问题。目前关于微生物中ARGs存在情况的研究主要集中在人类、家畜、医院或废水中。然而，工作场所和家庭灰尘抗性组中ARGs的种类相对未被充分探索。这项初步研究旨在分析参与者工作场所（一家儿科医院、一家妇产医院和一个研究中心）和家庭的室内灰尘样本中的抗性组，并比较两种不同的ARGs分析方法：高通量定量PCR（HT-qPCR）和全宏基因组鸟枪法测序（WMGS）。使用HT-qPCR总共检测到143种ARGs，其中与大环内酯类、林可酰胺类和链阳菌素B（MLSB）表型相关的ARGs最为丰富，其次是多药耐药（MDR）基因以及赋予对氨基糖苷类耐药性的基因。在工作场所的室内灰尘样本中观察到的ARGs总体相对数量高于家庭中的，其中儿科医院的ARGs相对数量最高。WMGS分析揭示了36种ARGs，其中5种通过HT-qPCR和WMGS技术均检测到。因此，WMGS检测ARGs的功效低于HT-qPCR。总之，我们的初步数据表明，人们大部分时间所在建筑物（工作场所、家庭）中的室内灰尘可能是抗微生物微生物的重要来源，这可能对人类和动物都构成健康风险。
