RECETOX, Faculty of Science, Masaryk University, Kotlarska 2, Brno, Czech Republic.
Department of Pediatric Otorhinolaryngology, University Hospital Brno, Černopolní 9, 613 00, Brno, Czech Republic.
BMC Microbiol. 2023 Jul 31;23(1):202. doi: 10.1186/s12866-023-02951-5.
Indoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments.
In this pilot study, nasopharyngeal swabs were taken from 22 participants in the morning and after 8 h of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants' households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital - NEO, 6 from a pediatric hospital - ENT, and 8 from a research center - RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices.
Staphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 h of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76% of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These "enriching" bacterial genera overlapped between the hospital workplaces - NEO and ENT but differed from those in the research center - RCX.
The results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day. Short-term exposure to the indoor environment can result in the enrichment of the nasopharynx with bacterial DNA from indoor dust; the bacterial composition, however, varies by the indoor workplace environment.
室内灰尘颗粒是人类日常接触微生物的来源，吸入这些微生物可能会直接影响呼吸道的微生物群。我们旨在描述人类鼻咽细菌组在短期暴露于室内（工作场所）环境后的变化。
在这项初步研究中，在早上和参与者在工作场所停留 8 小时后，分别从 22 名参与者的鼻咽部采集拭子。同时，从参与者的家庭（16 个来自公寓，6 个来自住宅）和工作场所（8 个来自产科医院-NEO，6 个来自儿科医院-ENT，8 个来自研究中心-RCX）采集室内灰尘样本。对这些人体和环境样本进行 16S rRNA 测序分析。
葡萄球菌属和棒状杆菌属是室内灰尘和鼻咽样本中最丰富的属。分析表明，与住宅、NEO、ENT 和 RCX 相比，公寓室内灰尘样本中的细菌多样性较低（p<0.05）。在所有组中，在 NEO 工作的参与者的鼻咽部细菌多样性最高（p<0.05）。暴露于工作场所环境 8 小时后，在 76%的研究参与者中观察到室内灰尘中存在的几种新细菌属在鼻咽部富集；然而，鼻咽部细菌多样性没有显著变化（p>0.05，Shannon 指数）。这些“富集”的细菌属在医院工作场所-NEO 和 ENT 之间重叠，但与研究中心-RCX 不同。
研究结果表明，尽管白天鼻咽细菌组的组成相对稳定，但短期暴露于室内环境可导致鼻咽部富集室内灰尘中的细菌 DNA；然而，细菌组成因室内工作场所环境而异。
