Xu Veena Wenqing, Yin Iris Xiaoxue, Niu John Yun, Yu Ollie Yiru, Nizami Mohammed Zahedul Islam, Chu Chun-Hung
Faculty of Dentistry, University of Hong Kong, Hong Kong, China.
Department of Mineralized Tissue Biology and Bioengineering, The ADA Forsyth Institute, Cambridge, MA, USA.
J Appl Biomater Funct Mater. 2024 Jan-Dec;22:22808000241307803. doi: 10.1177/22808000241307803.
A stable copper tetraamine fluoride (CTF) with low cytotoxicity has been developed for dental use.
To investigate the antimicrobial effects of CTF against common microbes associated with dental caries and periodontal disease.
The minimum inhibitory concentrations (MIC) and minimum bactericidal/fungicidal concentrations (MBC/MFC) were used to evaluate the antimicrobial effects of CTF against eight common bacteria and one fungus associated with dental caries and periodontal disease. These nine microbes included cariogenic pathogens (, , , , and ), pulpitis-related bacteria () and periodontal disease-related bacteria ( and ). Transmission electron microscopy (TEM) was employed to examine the morphological changes of microbes with and without CTF treatment.
The MIC of CTF against nine microbes ranged from 80 ppm () to 640 ppm ( and ). The MBC/MFC ranged from 320 ppm () to 2560 ppm (). TEM revealed abnormal curvature of cell membranes, disrupted cell membranes, cytoplasmic clear zone, and cytoplasmic content leakage of the microbes treated with CTF.
CTF has antimicrobial effects against common oral pathogens and presents a promising antimicrobial agent to aid management of dental caries and periodontal disease.
已开发出一种具有低细胞毒性的稳定四胺氟化铜（CTF）用于牙科。
研究CTF对与龋齿和牙周疾病相关的常见微生物的抗菌作用。
使用最低抑菌浓度（MIC）和最低杀菌/杀真菌浓度（MBC/MFC）来评估CTF对与龋齿和牙周疾病相关的八种常见细菌和一种真菌的抗菌作用。这九种微生物包括致龋病原体（、、、、和）、牙髓炎相关细菌（）和牙周疾病相关细菌（和）。采用透射电子显微镜（TEM）检查经CTF处理和未处理的微生物的形态变化。
CTF对九种微生物的MIC范围为80 ppm（）至640 ppm（和）。MBC/MFC范围为320 ppm（）至2560 ppm（）。TEM显示经CTF处理的微生物细胞膜出现异常弯曲、细胞膜破坏、细胞质透明区和细胞质内容物泄漏。
CTF对常见口腔病原体具有抗菌作用，是一种有助于管理龋齿和牙周疾病的有前景的抗菌剂。
