Zhou Jing, Ye Yibin, Chen Zhipeng, Liu Yong, Wu Baozheng, Huang Haiping
Department of Critical Care Medicine, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, Fujian, China.
Department of cerebrovascular Intervention Intensive Care Unit, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, Fujian, China.
Immun Inflamm Dis. 2024 Dec;12(12):e70113. doi: 10.1002/iid3.70113.
Sepsis-associated acute kidney injury (SA-AKI) is a common complication that can lead to renal failure in patients, significantly affecting the prognosis and survival of patients.
In this study, we aimed to evaluate the predictive value of NOD-like receptor protein 3 (NLRP3) and interleukin 18 (IL-18) in peripheral blood mononuclear cells (PBMCs) of SA patients for the occurrence of SA-AKI.
We screened AKI-related data sets using the Gene Expression Omnibus (GEO) database and identified differentially expressed genes (DEGs) associated with AKI. KEGG and GO analysis were used to identify enriched molecular functions and pathways. The study included 62 SA patients admitted to the Department of Intensive Care Medicine of our hospital from February 2021 to June 2022, including 34 non-AKI cases and 28 AKI cases, and 25 healthy volunteers were used as the control group. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the levels of NLRP3 and IL-18 in PBMCs of the subjects.
Bioinformatics analysis and experimental validation showed that the expression levels of NLRP3 and IL-18 were significantly upregulated in SA-AKI patients. In addition, the expressions of NLRP3 and IL-18 were positively correlated with APACHE II scores. ROC curve analysis revealed that NLRP3 and IL-18 have the potential to diagnose SA-AKI.
This study provides preliminary evidence for NLRP3 and IL-18 as potential diagnostic biomarkers for SA-AKI.
脓毒症相关急性肾损伤（SA-AKI）是一种常见并发症，可导致患者肾衰竭，显著影响患者的预后和生存。
本研究旨在评估脓毒症（SA）患者外周血单个核细胞（PBMCs）中NOD样受体蛋白3（NLRP3）和白细胞介素18（IL-18）对SA-AKI发生的预测价值。
我们使用基因表达综合数据库（GEO）筛选急性肾损伤（AKI）相关数据集，并鉴定与AKI相关的差异表达基因（DEGs）。采用KEGG和GO分析来鉴定富集的分子功能和途径。本研究纳入了2021年2月至2022年6月在我院重症医学科住院的62例SA患者，其中非AKI病例34例，AKI病例28例，并选取25名健康志愿者作为对照组。采用实时定量聚合酶链反应（RT-qPCR）检测受试者PBMCs中NLRP3和IL-18的水平。
生物信息学分析和实验验证表明，SA-AKI患者中NLRP3和IL-18的表达水平显著上调。此外，NLRP3和IL-18的表达与急性生理与慢性健康状况评分系统II（APACHE II）评分呈正相关。ROC曲线分析显示，NLRP3和IL-18具有诊断SA-AKI的潜力。
本研究为NLRP3和IL-18作为SA-AKI潜在诊断生物标志物提供了初步证据。
