Portz Tatiane Martinazzo, Mioranza Thaisa Muriel, Tartaro Eloisa Lorenzetti, Stangarlin José Renato, Kuhn Odair José
Department of Agronomy, Universidade Estadual Do Oeste Do Paraná, Marechal Cândido Rondon, PR, Brazil.
Arch Microbiol. 2024 Dec 18;207(1):16. doi: 10.1007/s00203-024-04213-y.
This work aimed to get compost with cellulose-rich residues for Pycnoporus sanguineus basidiocarp production. Two isolates of P. sanguineus (Ps08 and Ps14) were grown in polypropylene bags containing sawdust of Eucalyptus sp. and rice bran in rates of zero, 5% and 20%, and humidity of 75%. We used two granulometries of sawdust, less than 500 µm and between 500 and 841 µm, compress to get a density of 0.5 g mL. Basidiocarps were harvested at 90 and 180 days of incubation, evaluating quantity, diameter, fresh and dry masses and production of the pigment cinnabarin. For the variable granulometry, there was an effect only for the isolate Ps14, which produced basidiocarps with diameters 14.65 mm in granulometry less than 500 µm and 8.56 in granulometry 500-841 µm, however, comparing the isolates, Ps08 produced larger basidiocarps, with an average diameter of 36.99 mm while 11.60 mm for Ps14. Only the isolate Ps08 responded in a dose-dependent manner to rice bran concentrations. As for the fresh mass of basidiocarps, in both harvests the isolate Ps08 presented higher values than the isolate Ps14 in the first and second harvests, in the granulometry less than 500 µm. The cinnabarin content of Ps14 isolate was higher than Ps08, with values of 0.67 e 0.43 mg mL, respectively, in the granulometry 500-841 µm, with a tendency to increase in cinnabarin content with higher additions of rice bran. Thus, it was possible to produce basidiocarps of P. sanguineus in an axenic way using cellulosic residues supplemented with rice bran.
这项工作旨在利用富含纤维素的残渣获得堆肥,用于生产血红密孔菌子实体。将两株血红密孔菌(Ps08和Ps14)接种在聚丙烯袋中,袋中含有桉木锯末和米糠,米糠添加比例分别为零、5%和20%,湿度为75%。我们使用了两种粒度的锯末,小于500微米和500至841微米之间,压缩后密度达到0.5克/毫升。在培养90天和180天时收获子实体,评估其数量、直径、鲜重和干重以及色素朱红菌素的产量。对于粒度变量,仅对菌株Ps14有影响,其在粒度小于500微米时产生的子实体直径为14.65毫米,在粒度500 - 841微米时为8.56毫米,然而,比较这两个菌株,Ps08产生的子实体更大,平均直径为36.99毫米,而Ps14为11.60毫米。只有菌株Ps08对米糠浓度呈剂量依赖性反应。至于子实体的鲜重,在两次收获中,在粒度小于500微米时,菌株Ps08在第一次和第二次收获时的值均高于菌株Ps14。菌株Ps14的朱红菌素含量高于Ps08,在粒度500 - 841微米时分别为0.67和0.43毫克/毫升,随着米糠添加量增加,朱红菌素含量有增加趋势。因此,利用添加米糠的纤维素残渣以无菌方式生产血红密孔菌子实体是可行的。