Meng Di, Shao Xuan, Luo Shou-Peng, Tian Qiao-Peng, Liao Xiang-Ru
Henan Provincial Engineering Research Center for Development and Application of Characteristic Microorganism Resources, Shangqiu Normal University, Shangqiu, China.
Hua Tian Engineering & Technology Corporation, MCC, Nanjing, China.
Front Microbiol. 2022 Oct 19;13:1015913. doi: 10.3389/fmicb.2022.1015913. eCollection 2022.
Natural pigments are playing important roles in our daily lives. They not only make products colorful but also provide various health benefits for humans. In addition, genus, listed as food- and cosmetic-grade microorganism, is one of the promising organisms for developing natural pigments. In this study, a new fungal strain with high efficiency in producing intense orange pigments was isolated and identified as SYBC-L7. Different agro-industrial wastes were applied to evaluate the growth and pigment production of strain SYBC-L7. SYBC-L7 can grow rapidly and effectively produce pigments using wood chips as substrate in solid-state fermentation (SSF). Culture conditions were also optimized for value-added pigments production and the optimum production conditions were glucose as carbon source, ammonium tartrate as nitrogen source, initial pH 6.0, and relative humidity of 65%. Pigment components, cinnabarinic acid, tramesanguin, and 2-amino-9-formylphenoxazone-1-carbonic acid were confirmed by liquid chromatography-mass spectrometry. Meanwhile, an agar plate diffusion assay was performed to evaluate the antimicrobial activity of the pigment. These pigments showed more significant inhibition of Gram-positive than Gram-negative bacteria. The results showed that SYBC-L7 was able to cost-effectively produce intense natural orange pigments with antibacterial activity in SSF, which is the basis of their large-scale production and application.
天然色素在我们的日常生活中发挥着重要作用。它们不仅使产品色彩丰富，还为人类提供了各种健康益处。此外，被列为食品和化妆品级微生物的[具体属名未给出]属，是开发天然色素的有前景的生物体之一。在本研究中，分离出了一种高效生产强烈橙色色素的新真菌菌株，并鉴定为SYBC-L7。使用不同的农业工业废弃物来评估菌株SYBC-L7的生长和色素生产情况。SYBC-L7能够在固态发酵（SSF）中以木屑为底物快速生长并有效生产色素。还针对增值色素生产对培养条件进行了优化，最佳生产条件为以葡萄糖作为碳源、酒石酸铵作为氮源、初始pH值为6.0以及相对湿度为65%。通过液相色谱-质谱法确认了色素成分，包括朱红酸、tramesanguin和2-氨基-9-甲酰基吩恶嗪-1-碳酸。同时，进行了琼脂平板扩散试验以评估色素的抗菌活性。这些色素对革兰氏阳性菌的抑制作用比对革兰氏阴性菌更为显著。结果表明，SYBC-L7能够在固态发酵中经济高效地生产具有抗菌活性的强烈天然橙色色素，这是其大规模生产和应用的基础。
