Shah Muhammad Tariq, Alveroglu Esra
Faculty of Science and Letters, Department of Physics Engineering, Istanbul Technical University, Istanbul, Maslak, 34469, Turkey.
Higher Education Department, Khyber Pakhtunkhwa, Government Degree College Tangi, District Charsadda, 24540, Pakistan.
J Fluoresc. 2024 Dec 18. doi: 10.1007/s10895-024-04084-y.
This paper describes the synthesis of CoFe₂O₄ nanoparticles via a simple ultrasonic-assisted co-precipitation method and their functionalization with thiol groups using (3-Mercaptopropyl)trimethoxysilane (MPTS) as the functionalizing agent. The use of ultrasonic energy not only serves as a green energy source but also reduces the reaction time fivefold compared to conventional methods. The synthesized CoFe₂O₄ nanoparticles were characterized for their surface and internal properties using instrumental techniques such as Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Vibrating Sample Magnetometer (VSM). The functionalized nanoparticles were applied for the effective adsorption of bovine serum albumin (BSA) from a buffered aqueous medium. To enhance the adsorption performance, the influence of pH, amount of solid adsorbent, initial BSA concentration, shaking time, and temperature on protein adsorption was investigated. Lagergren pseudo-second-order adsorption kinetics fitted the BSA adsorption data well, with an acceptable R² value of 0.976. Additionally, the BSA adsorption data were analyzed using Langmuir and Freundlich isotherm models, and it was concluded that the experimental data followed the Langmuir equation more closely than the Freundlich equation. At an initial pH of 5.57 (acetate buffer), the adsorption capacity (Q₀) for BSA protein was found to be 200 mg/g. The thermodynamic study revealed ΔS° and ΔH° values of 17.40 J/mol·K and - 45.37 kJ/mol, respectively.
本文描述了通过简单的超声辅助共沉淀法合成CoFe₂O₄纳米颗粒，并使用（3-巯丙基）三甲氧基硅烷（MPTS）作为功能化剂对其进行巯基功能化。超声能量的使用不仅作为一种绿色能源，而且与传统方法相比，反应时间缩短了五倍。使用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）和振动样品磁强计（VSM）等仪器技术对合成的CoFe₂O₄纳米颗粒的表面和内部性质进行了表征。将功能化的纳米颗粒用于从缓冲水性介质中有效吸附牛血清白蛋白（BSA）。为了提高吸附性能，研究了pH值、固体吸附剂用量、初始BSA浓度、振荡时间和温度对蛋白质吸附的影响。Lagergren准二级吸附动力学很好地拟合了BSA吸附数据，可接受的R²值为0.976。此外，使用Langmuir和Freundlich等温线模型对BSA吸附数据进行了分析，得出实验数据更符合Langmuir方程而非Freundlich方程的结论。在初始pH值为5.57（醋酸盐缓冲液）时，发现BSA蛋白的吸附容量（Q₀）为200 mg/g。热力学研究表明，ΔS°和ΔH°值分别为17.40 J/mol·K和 - 45.37 kJ/mol。
