Thompson B J, Smith S
Pediatr Res. 1985 Jan;19(1):139-43. doi: 10.1203/00006450-198501000-00036.
The objective of this study was to characterize the fatty acid biosynthetic pathway of the lactating human breast. Mixed cell populations, obtained by centrifugation of human milk, were enriched in breast epithelial cells by a selective adsorption procedure. Confirmation of the identity of the breast epithelial cells was obtained immunohistochemically. These viable breast epithelial cells incorporated radioactively labeled acetate predominantly into fatty acids with less than 16C atoms. The presence of the two key enzymes characteristic of the medium-chain fatty acid biosynthetic pathway of nonruminants, fatty acid synthetase, and thioesterase II, was demonstrated both qualitatively, by immunohistochemistry, and quantitatively, by enzyme assay. The results indicate that the lipogenic system of the human breast is qualitatively very similar to that of rats, mice, and rabbits, which also secrete milk fats containing medium-chain fatty acids. Quantitatively, however, the mammary fatty acid biosynthetic pathway appears to be less active in humans than in these other species.
本研究的目的是表征哺乳期人类乳腺的脂肪酸生物合成途径。通过对人乳进行离心获得的混合细胞群体，经选择性吸附程序富集乳腺上皮细胞。通过免疫组织化学方法确认了乳腺上皮细胞的身份。这些活的乳腺上皮细胞将放射性标记的乙酸盐主要掺入碳原子数少于16的脂肪酸中。通过免疫组织化学定性和酶测定法定量证明了非反刍动物中链脂肪酸生物合成途径的两种关键酶，脂肪酸合成酶和硫酯酶II的存在。结果表明，人类乳腺的脂肪生成系统在性质上与大鼠、小鼠和兔子的非常相似，它们也分泌含有中链脂肪酸的乳脂肪。然而，从数量上看，人类乳腺脂肪酸生物合成途径的活性似乎低于这些其他物种。
