Wang Qianru, Li Qingmei, Ouyang Fangzhu, Ke Bixia, Jiang Shiqin, Liu Jiajun, Yan Jin, Li Baisheng, Tan Wei, He Dongmei
Department of Microbiological Laboratory, Baoan District Center for Disease Control and Prevention, Shenzhen 518101, China.
Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Shenzhen Medical Academy of Research and Translation (SMART), Shenzhen 518107, China.
Int J Food Microbiol. 2025 Feb 2;429:111025. doi: 10.1016/j.ijfoodmicro.2024.111025. Epub 2024 Dec 15.
The Pearl River Delta (PRD) region in southern China is a densely populated area and a hotspot for Vibrio parahaemolyticus infections. However, systematic research on this pathogen, particularly comparing clinical and environmental strains, remains limited. This study analyzed the molecular epidemiology and antimicrobial resistance of 200 V. parahaemolyticus isolates from 12 cities in the PRD region from 2022 to 2023. The results indicated that the most prevalent serotypes were O3:K6 (39.5 %) and O10:K4 (27.5 %), predominantly found in clinical isolates. Most clinical isolates exhibited the characteristics of toxRS/new, tdh, and trh, along with the sequence type 3 (ST3), while environmental isolates did not possess these genetic markers. Antimicrobial susceptibility testing showed that although clinically recommended antibiotics remain effective, some isolates have exhibited resistance, with environmental isolates displaying higher rates of antimicrobial resistance than clinical isolates. Moreover, a total of 26 antibiotic resistance genes (ARGs) associated with 10 antibiotic categories were identified, showing variations in distribution patterns among isolates from different sources. Phylogenetic analysis indicated that clinical isolates formed a distinct lineage, contrasting with the greater diversity observed in environmental isolates. Whole-genome analysis further revealed significant differences in pathogenicity-related genes between the two groups, with genes associated with biofilm formation and antimicrobial resistance being more commonly found in environmental isolates. These findings underscore the genetic variability and distinct patterns of antimicrobial resistance between clinical and environmental V. parahaemolyticus strains, highlighting the need for ongoing surveillance and targeted interventions to effectively address foodborne illnesses.
中国南方的珠江三角洲（PRD）地区人口密集，是副溶血性弧菌感染的热点地区。然而，对这种病原体的系统研究，特别是对临床菌株和环境菌株的比较研究仍然有限。本研究分析了2022年至2023年从珠江三角洲地区12个城市分离出的200株副溶血性弧菌的分子流行病学和抗菌药物耐药性。结果表明，最常见的血清型是O3:K6（39.5%）和O10:K4（27.5%），主要存在于临床分离株中。大多数临床分离株表现出toxRS/new、tdh和trh的特征，以及序列类型3（ST3），而环境分离株则不具备这些遗传标记。抗菌药物敏感性测试表明，虽然临床推荐的抗生素仍然有效，但一些分离株已经表现出耐药性，环境分离株的抗菌药物耐药率高于临床分离株。此外，共鉴定出与10类抗生素相关的26个抗生素耐药基因（ARGs），不同来源的分离株在分布模式上存在差异。系统发育分析表明，临床分离株形成了一个独特的谱系，与环境分离株中观察到的更大的多样性形成对比。全基因组分析进一步揭示了两组之间致病性相关基因的显著差异，与生物膜形成和抗菌药物耐药性相关的基因在环境分离株中更常见。这些发现强调了临床和环境副溶血性弧菌菌株之间的遗传变异性和抗菌药物耐药性的不同模式，突出了持续监测和针对性干预以有效应对食源性疾病的必要性。
