Baheshm Yasser A, Zayed Alia, Awash Abdullah A, Follis Madison, Terreri Payton, Samake Jeanne N, Aljasari Adel, Harwood James F, Lenhart Audrey, Zohdy Sarah, Al-Eryani Samira M, Carter Tamar E
National Malaria Control Program, Ministry of Health, Aden, Yemen.
US Naval Medical Research Unit-EURAFCENT (Previously NAMRU-3), Cairo Detachment, Cairo , Egypt.
Parasit Vectors. 2024 Dec 18;17(1):507. doi: 10.1186/s13071-024-06601-1.
The invasion of Anopheles stephensi in Africa warrants investigation of neighboring countries. In this study, genetic analysis was applied to determine the status of An. stephensi in southern Yemen. Cytochrome c oxidase subunit I (COI) and internal transcribed spacer 2 (ITS2) were sequenced in An. stephensi collected in Dar Sa'ad (Aden City), Tuban, Rodoom, Al Mukalla, and Sayhut, and phylogenetic analysis confirmed An. stephensi identity. Our analyses revealed that the ITS2 sequences were identical in all An. stephensi, while COI analysis revealed two haplotypes, one previously reported in northern Horn of Africa and one identified in this study for the first time. Overall, these findings revealed low levels of mitochondrial DNA diversity, which is consistent with a more recent population introduction in parts of southern Yemen relative to the Horn of Africa. Further, whole genomic analysis is needed to elucidate the original connection with invasive populations of An. stephensi in the Horn of Africa.
斯氏按蚊侵入非洲，这使得对周边国家展开调查很有必要。在本研究中，运用了基因分析来确定也门南部斯氏按蚊的状况。对在萨阿德港（亚丁市）、图班、罗杜姆、穆卡拉和赛胡特采集的斯氏按蚊进行了细胞色素c氧化酶亚基I（COI）和内部转录间隔区2（ITS2）测序，系统发育分析证实了斯氏按蚊的身份。我们的分析表明，所有斯氏按蚊的ITS2序列均相同，而COI分析揭示了两种单倍型，一种先前在非洲之角北部有报道，另一种是本研究首次鉴定出的。总体而言，这些发现揭示了线粒体DNA多样性水平较低，这与也门南部部分地区相对于非洲之角而言，斯氏按蚊是较近期引入的种群这一情况相符。此外，需要进行全基因组分析，以阐明与非洲之角斯氏按蚊入侵种群的原始联系。
