Ahmed Dalia, Abdel-Shafy Ebtesam A, Mohammed Elsadig Ahmed Adam, Alnour Husam Elden Alnour Bakhet, Ismail Amar Mohamed, Cacciatore Stefano, Zerbini Luiz Fernando
Department of Histopathology and Cytology, Faculty of Medical Laboratory Science, Al-Neelain University, Khartoum - Sudan.
Bioinformatics Unit, International Centre for Genetic Engineering and Biotechnology, Cape Town - South Africa.
J Circ Biomark. 2024 Dec 16;13:36-44. doi: 10.33393/jcb.2024.3146. eCollection 2024 Jan-Dec.
Prostate cancer (PCa) management presents a multifaceted clinical challenge, intricately linking oncological considerations with cardiovascular health. Despite the recognized importance of lipid metabolism and hypertension in this interwoven relationship, their involvement in PCa development remains partially understood. This study aimed to explore variations in plasma metabolome among Sudanese PCa patients and their associated comorbidities.
Plasma samples were collected from 50 patients across four hospitals in Sudan and profiled by nuclear magnetic resonance (NMR) spectroscopy. One-dimensional proton NMR spectra were acquired for each sample using standard nuclear Overhauser effect spectroscopy pulse sequence presat on a 500 MHz Bruker Avance III HD NMR spectrometer. Metabolite concentrations were quantified using R scripts developed in-house. Univariate and multivariate analyses were generated in the R software.
Patients were categorized into four distinct metabotypes based on their metabolic profiles, and statistical analyses were conducted to evaluate the significance of observed differences. Our findings revealed high levels of fatty acids, phospholipids, cholesterol, valine, leucine, and isoleucine associated with non-hypertensive patients. In contrast, hypertensive patients were associated with high GlycA and GlycB levels and altered amino acid metabolism.
These findings underscore the intricate interplay between metabolic dysregulation and hypertension in PCa patients. Further research is warranted to elucidate the precise molecular pathways underlying lipid metabolism in PCa and to explore the therapeutic potential of targeting these pathways. In conclusion, our study contributes to a deeper understanding of the metabolic landscape of PCa in Sudanese patients, emphasizing the importance of personalized approaches in cancer management.
前列腺癌（PCa）的管理是一项多方面的临床挑战，将肿瘤学考量与心血管健康复杂地联系在一起。尽管脂质代谢和高血压在这种交织关系中的重要性已得到认可，但其在PCa发展中的作用仍部分不明确。本研究旨在探索苏丹PCa患者血浆代谢组的变化及其相关合并症。
从苏丹四家医院的50名患者中采集血浆样本，并用核磁共振（NMR）光谱进行分析。使用标准的核Overhauser效应光谱预饱和脉冲序列，在500 MHz布鲁克Avance III HD NMR光谱仪上为每个样本采集一维质子NMR光谱。使用内部开发的R脚本对代谢物浓度进行定量。在R软件中进行单变量和多变量分析。
根据代谢谱将患者分为四种不同的代谢型，并进行统计分析以评估观察到的差异的显著性。我们的研究结果显示，非高血压患者的脂肪酸、磷脂、胆固醇、缬氨酸、亮氨酸和异亮氨酸水平较高。相比之下，高血压患者的GlycA和GlycB水平较高，且氨基酸代谢发生改变。
这些发现强调了PCa患者代谢失调与高血压之间的复杂相互作用。有必要进一步研究以阐明PCa中脂质代谢的精确分子途径，并探索靶向这些途径的治疗潜力。总之，我们的研究有助于更深入地了解苏丹患者PCa的代谢情况，强调个性化方法在癌症管理中的重要性。
