Vedtofte P, Pindborg J J, Hakomori S
Acta Pathol Microbiol Immunol Scand A. 1985 Jan;93(1):25-34. doi: 10.1111/j.1699-0463.1985.tb03916.x.
The distribution of epithelial cell surface antigens was studied in normal odontogenic epithelium from 20 fetuses and in odontogenic epithelium from 15 ameloblastomas, 16 odontogenic keratocysts, 15 follicular and 15 radicular cysts. The cell surface carbohydrates were detected using antibodies with reactivity for the blood group antigens A, B, H type 2 (A and B precursor) and N-acetyllactosamine (N-lac, H type 2 precursor) by an immunofluorescence technique. The expression of the blood group carbohydrates differed considerably in normal fetal odontogenic epithelium from that in ameloblastomas and odontogenic cysts. The A, B and H type 2 antigens were demonstrated in odontogenic keratocysts and in follicular and radicular cysts. Expression of the blood group carbohydrates was similar in follicular and radicular cysts but differed from that seen in odontogenic keratocysts by the failure to detect N-lac in the latter. The antigens A, B, H type 2 and N-lac were not expressed in any of the ameloblastomas including types with palisading of basal cells and polarization of basal cell nuclei and types with a plexiform pattern with cuboidal or polyhedral shaped peripheral cells. The findings indicate that epithelium of ameloblastomas can be distinguished from odontogenic cyst epithelium by differences in expression of cell surface carbohydrates with blood group specificity.
研究了20例胎儿正常牙源性上皮以及15例成釉细胞瘤、16例牙源性角化囊肿、15例滤泡囊肿和15例根端囊肿的牙源性上皮中上皮细胞表面抗原的分布情况。采用免疫荧光技术，使用对血型抗原A、B、H2型（A和B的前体）以及N-乙酰乳糖胺（N-lac，H2型前体）具有反应性的抗体来检测细胞表面碳水化合物。正常胎儿牙源性上皮中血型碳水化合物的表达与成釉细胞瘤和牙源性囊肿中的表达有很大差异。A、B和H2型抗原在牙源性角化囊肿以及滤泡囊肿和根端囊肿中均有显示。滤泡囊肿和根端囊肿中血型碳水化合物的表达相似，但与牙源性角化囊肿不同，后者未检测到N-lac。包括具有基底细胞栅栏状排列和基底细胞核极化的类型以及具有由立方状或多面体外周细胞组成的丛状结构的类型在内的任何成釉细胞瘤中均未表达A、B、H2型抗原和N-lac。这些发现表明，成釉细胞瘤的上皮可以通过具有血型特异性的细胞表面碳水化合物表达差异与牙源性囊肿上皮区分开来。
