Vedtofte P, Dabelsteen E, Hakomori S, Young W W
Differentiation. 1984;25(3):221-8. doi: 10.1111/j.1432-0436.1984.tb01360.x.
The distribution of blood group carbohydrate chains with antigen A, B, H type 2 chain (A and B precursor), and N-acetyllactosamine (H type 2 precursor) specificity was studied in human oral epithelium from different anatomical regions. These represented various epithelial differentiation patterns such as non-keratinized, parakeratinized, and orthokeratinized stratified squamous epithelium. The material included buccal and palatal epithelium from 20 persons with blood group A or O, gingival, and alveolar epithelium from 10 persons with blood group A or B, and buccal metaplastically keratinized epithelium from nine blood group A, two blood group B, and nine blood group O individuals. The blood group carbohydrate chains were examined in tissue sections by immunofluorescence microscopy. The A and B blood group antigens were detected by human blood group sera, and antigen H type 2 chains and N-acetyllactosamine by murine monoclonal antibodies. Each antigen showed a similar staining pattern in buccal and alveolar epithelium (non-keratinized) which differed considerably from that seen in palatal and gingival epithelium (ortho- and parakeratinized). The expression of blood group antigens A or B and the precursor antigen H type 2 chains in metaplastically keratinized buccal epithelium was found to differ significantly from that seen in normal non-keratinized buccal epithelium. The regional variations demonstrated in cell surface carbohydrates are suggested to reflect differences in tissue differentiation.
研究了具有抗原A、B、H2型链（A和B前体）以及N-乙酰乳糖胺（H2型前体）特异性的血型碳水化合物链在不同解剖区域的人口腔上皮中的分布。这些区域代表了各种上皮分化模式，如非角化、不全角化和正角化的复层鳞状上皮。材料包括来自20名A型或O型血型者的颊黏膜和腭黏膜上皮、来自10名A型或B型血型者的牙龈和牙槽上皮，以及来自9名A型、2名B型和9名O型血型者的颊黏膜化生角化上皮。通过免疫荧光显微镜检查组织切片中的血型碳水化合物链。用人类血型血清检测A和B血型抗原，用鼠单克隆抗体检测H2型链抗原和N-乙酰乳糖胺。每种抗原在颊黏膜和牙槽上皮（非角化）中呈现相似的染色模式，这与腭黏膜和牙龈上皮（正角化和不全角化）中的染色模式有很大不同。发现化生角化颊黏膜上皮中血型抗原A或B以及前体抗原H2型链的表达与正常非角化颊黏膜上皮中的表达有显著差异。细胞表面碳水化合物的区域差异表明反映了组织分化的差异。
