Tang Rong, Gui Xingyu, Han Ruiying, Gao Canyu, Zhang Hui, Lu Shengkai, Zhao Junyu, Zhou Weikai, Chen Axuan, Sun Huan, Sun Jianxun, Zhai Yun, Zhao Zhihe, Zhou Changchun
State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan, China.
National Engineering Research Center for Biomaterials, College of Biomedical Engineering, Sichuan University, Chengdu 610064, Sichuan, China.
J Mater Chem B. 2025 Jan 29;13(5):1712-1730. doi: 10.1039/d4tb02242d.
Alveolar bone defects are often irregular in shape and can severely affect patients' physical and psychological well-being, posing significant challenges in treatment, particularly in cases complicated by systemic diseases. This study presents a shape-adaptive hydrogel with sequential antibacterial and osteogenic functions designed to repair irregular bone defects associated with osteoporosis. Naringin, an estrogen analogue, was conjugated to the hydrogel disulfide bonds and then uniformly mixed with nano-hydroxyapatite (nano-HAP) to create microspheres. These microspheres were uniformly dispersed within the naringin-loaded hydrogel, forming an injectable and photocurable suspension. Upon implantation, naringin is rapidly released due to diffusion along the concentration gradient and initial hydrogel degradation, providing antibacterial effects and preventing infection. As bone repair progresses, the hydrogel undergoes further degradation and the disulfide bonds break, so that naringin is continuously released, which enhances osteoblast differentiation and inhibits osteoclast differentiation. Material characterization confirmed the presence of disulfide bonds and the sustained release profile of naringin. Both and experiments demonstrated the hydrogel's excellent biocompatibility and its effectiveness in repairing regular mandibular defects as well as irregular alveolar bone defects associated with osteoporosis. This hydrogel provides a promising strategy for the development of advanced biomaterials tailored to the complex requirements of irregular bone defect repair under osteoporotic conditions.
牙槽骨缺损通常形状不规则，会严重影响患者的身心健康，给治疗带来重大挑战，尤其是在合并全身性疾病的情况下。本研究提出了一种具有顺序抗菌和成骨功能的形状适应性水凝胶，旨在修复与骨质疏松症相关的不规则骨缺损。柚皮苷是一种雌激素类似物，通过与水凝胶二硫键共轭，然后与纳米羟基磷灰石（nano-HAP）均匀混合以制备微球。这些微球均匀分散在负载柚皮苷的水凝胶中，形成可注射且可光固化的悬浮液。植入后，柚皮苷由于沿浓度梯度扩散和初始水凝胶降解而迅速释放，提供抗菌作用并预防感染。随着骨修复的进行，水凝胶进一步降解且二硫键断裂，使得柚皮苷持续释放，从而增强成骨细胞分化并抑制破骨细胞分化。材料表征证实了二硫键的存在以及柚皮苷的持续释放特性。体内和体外实验均证明了该水凝胶具有出色的生物相容性，以及在修复规则下颌骨缺损和与骨质疏松症相关的不规则牙槽骨缺损方面的有效性。这种水凝胶为开发针对骨质疏松条件下不规则骨缺损修复复杂需求的先进生物材料提供了一种有前景的策略。
