Li Xinyi, Chen Bingjie, Xie Yaoyao, Luo Yi, Zhu Dan, Wang Lianhui, Su Shao
State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory of Smart Biomaterials and Theranostic Technology, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.
Anal Chem. 2025 Jan 14;97(1):793-798. doi: 10.1021/acs.analchem.4c05329. Epub 2024 Dec 19.
The early diagnosis of Alzheimer's disease (AD) plays a vital role in slowing the progression of AD and improving the quality of human life. However, it is still a challenge in the medical field. Herein, an ultrasensitive fluorescent aptasensor was designed for the detection of special phosphorylated tau181 (P-tau181) by coupling with polyvalent aptamers (PAs) structure, hybridization chain reaction (HCR), and the CRISPR-Cas system. Coupling with the signal amplification strategy, the specific recognition ability of the aptamer, and the high cleavage activity of Cas12a protein, the designed aptasensor showed a wide linear range (0.1-10 pg/mL), a low detection limit (0.069 pg/mL), high selectivity, and excellent anti-interference ability for the detection of P-tau181. Moreover, the aptasensor can efficiently analyze P-tau181 in artificial cerebro spinal fluid (aCSF) and serum, proving that it has a promising application in the early diagnosis of AD.
阿尔茨海默病（AD）的早期诊断对于减缓AD的进展和提高人类生活质量起着至关重要的作用。然而，这在医学领域仍然是一项挑战。在此，通过与多价适体（PAs）结构、杂交链式反应（HCR）和CRISPR-Cas系统相结合，设计了一种超灵敏荧光适体传感器，用于检测特殊的磷酸化tau181（P-tau181）。结合信号放大策略、适体的特异性识别能力和Cas12a蛋白的高切割活性，所设计的适体传感器在检测P-tau181时表现出宽线性范围（0.1-10 pg/mL）、低检测限（0.069 pg/mL）、高选择性和出色的抗干扰能力。此外，该适体传感器能够有效分析人工脑脊液（aCSF）和血清中的P-tau181，证明其在AD早期诊断中具有广阔的应用前景。
