Yuan Meng, Fang Xiao, Liu Wenzheng, Ge Xiaoguang, Wu Ying, Su Lichao, Gao Shi, Song Jibin
Key BioAI Synthetica Lab for Natural Product Drug Discovery, College of Bee and Biomedical Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China.
Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China, MOE Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China.
ACS Appl Bio Mater. 2025 Jan 20;8(1):368-373. doi: 10.1021/acsabm.4c01352. Epub 2024 Dec 19.
Afterglow fluorescence imaging has been extensively assessed in ultrasensitive bioimaging. Since it eliminates the need for real-time excitation light and thereby circumvents the autofluorescence background of tissue, it holds tremendous potential in accurate biomedical imaging. However, current afterglow probes are rare and emit light only in the visible to near-infrared (NIR) range, which is inadequate for imaging. To resolve this issue, an ultrasound (US)-activated NIR-II afterglow luminescence probe (NPs-Ce4-SN) emitting afterglow luminescence with a peak at ∼1100 nm was developed. This peak is nearly 400 nm red-shifted compared with other reported afterglow probes. Of note, after US termination, NPs-Ce4-SN undergoes energy transformation to produce O and subsequently undergoes internal oxidation-reduction reaction to produce NIR-II afterglow, generating high signal-to-noise ratio and high-penetration depth imaging. and NIR-II afterglow imaging experiments revealed that NPs-Ce4-SN has good biocompatibility and deep tissue penetration depth, suggesting a diagnostic strategy for tumor imaging with a high signal-to-noise ratio.
余辉荧光成像已在超灵敏生物成像中得到广泛评估。由于它无需实时激发光，从而规避了组织的自发荧光背景，在精确生物医学成像方面具有巨大潜力。然而，目前的余辉探针很少见，且仅在可见光到近红外（NIR）范围内发光，这对于成像来说是不够的。为了解决这个问题，开发了一种超声（US）激活的近红外二区（NIR-II）余辉发光探针（NPs-Ce4-SN），其发射的余辉发光峰值在~1100 nm。与其他报道的余辉探针相比，这个峰值有近400 nm的红移。值得注意的是，在超声作用终止后，NPs-Ce4-SN发生能量转换产生O，随后进行内部氧化还原反应产生近红外二区余辉，从而实现高信噪比和高穿透深度成像。近红外二区余辉成像实验表明，NPs-Ce4-SN具有良好的生物相容性和深部组织穿透深度，为高信噪比肿瘤成像提供了一种诊断策略。
