Yan Shiqiang, Lu Yan, An Changming, Hu Wanglai, Chen Yaofeng, Li Ziwen, Wei Wenbo, Chen Zongzheng, Zeng Xianhai, Xu Wei, Lv Zhenghua, Pan Fan, Gao Wei, Wu Yongyan
Shenzhen Institute of Otolaryngology & Key Laboratory of Otolaryngology, Longgang Otolaryngology Hospital, Shenzhen 518172, Guangdong, China; Center of Cancer Immunology, Shenzhen Institute of Advanced Technology Chinese Academy of Sciences, Shenzhen 518055, Guangdong, China.
Department of Otolaryngology Head & Neck Surgery, The First Hospital, Jinzhou Medical University, Jinzhou 121001, Liaoning, China; Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, Department of Otolaryngology Head & Neck Surgery, First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, China.
J Adv Res. 2025 Oct;76:615-637. doi: 10.1016/j.jare.2024.12.024. Epub 2024 Dec 17.
Cells in the body reside in a dynamic microenvironment subjected to various physical stimuli, where mechanical stimulation plays a crucial role in regulating cellular physiological behaviors and functions.
Investigating the mechanisms and interactions of mechanical transmission is essential for understanding the physiological and functional interplay between cells and physical stimuli. Therefore, establishing an in vitro biomechanical stimulation cell culture system holds significant importance for research related to cellular biomechanics.
In this review, we primarily focused on various biomechanically relevant cell culture systems and highlighted the advancements and prospects in their preparation processes. Firstly, we discussed the types and characteristics of biomechanics present in the microenvironment within the human body. Subsequently, we introduced the research progress, working principles, preparation processes, potential advantages, applications, and challenges of various biomechanically relevant in vitro cell culture systems. Additionally, we summarized and categorized currently commercialized biomechanically relevant cell culture systems, offering a comprehensive reference for researchers in related fields.
体内细胞存在于受到各种物理刺激的动态微环境中，其中机械刺激在调节细胞生理行为和功能方面起着关键作用。
研究机械信号传导的机制及其相互作用对于理解细胞与物理刺激之间的生理和功能相互作用至关重要。因此，建立体外生物力学刺激细胞培养系统对于细胞生物力学相关研究具有重要意义。
在本综述中，我们主要关注各种与生物力学相关的细胞培养系统，并突出了其制备过程中的进展和前景。首先，我们讨论了人体微环境中存在的生物力学类型和特征。随后，我们介绍了各种与生物力学相关的体外细胞培养系统的研究进展、工作原理、制备过程、潜在优势、应用和挑战。此外，我们对目前商业化的与生物力学相关的细胞培养系统进行了总结和分类，为相关领域的研究人员提供了全面的参考。
