Garcia Andrés J, Gallant Nathan D
Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332-0363, USA.
Cell Biochem Biophys. 2003;39(1):61-73. doi: 10.1385/CBB:39:1:61.
Cell adhesion to extracellular matrix components involves integrin receptor-ligand binding and adhesion strengthening, comprising receptor clustering, cytoskeletal interactions, and cell spreading. Although elucidation of the biochemical events in adhesive interactions is rapidly advancing, the mechanical processes and mechanisms of adhesion strengthening remain poorly understood. Because the biochemical and biophysical processes in adhesive interactions are tightly coupled, mechanical analyses of adhesion strength provide critical information on structure-function relationships. This review focuses on (a) measurement systems for cell adhesion strength and (b) quantitative analyses of integrin-mediated strengthening to extracellular matrix components.
细胞与细胞外基质成分的黏附涉及整合素受体 - 配体结合以及黏附增强，包括受体聚集、细胞骨架相互作用和细胞铺展。尽管对黏附相互作用中生化事件的阐明正在迅速推进，但黏附增强的力学过程和机制仍知之甚少。由于黏附相互作用中的生化和生物物理过程紧密耦合，黏附强度的力学分析为结构 - 功能关系提供了关键信息。本综述重点关注（a）细胞黏附强度的测量系统，以及（b）整合素介导的对细胞外基质成分增强作用的定量分析。
