Fu Meng, Yu Jing-Jing, Yan Mei-Qiu, Su Jie, Zheng Jia-Yi, Hu Xue-Ling, Lyu Gui-Yuan, Chen Jian-Zhen
School of Pharmaceutical Sciences, Zhejiang Chinese Medical University Hangzhou 310053, China.
Zhongguo Zhong Yao Za Zhi. 2024 Oct;49(20):5577-5586. doi: 10.19540/j.cnki.cjcmm.20240715.301.
This study aims to reveal the effects and mechanisms of different fractions of Polygonati Rhizoma on the reproductive dysfunction in male mice with kidney essence deficiency due to excess of sexual intercourse. Fifty male ICR mice with good sexual function were selected and randomized into normal(NC), model(MC), n-butanol fraction of Polygonati Rhizoma(0.4 g·kg(-1), HJCT), remaining fraction of Polygonati Rhizoma(0.4 g·kg(-1), HJSY), and ultra-micro powder of Polygonati Rhizoma(1.0 g·kg~(-1), HJCW) groups. The Coolidge effect was used to induce excess of sexual intercourse in the mice, and a mouse model of reproductive dysfunction with kidney essence deficiency was established. The mice were administrated with corresponding drugs at the same time of modeling for 8 consecutive weeks. The anal temperature, grip strength, locomotor activities, weight-bearing swimming time, and sexual behaviors(catching, straddling, and ejaculation) were measured. Enzyme-linked immunosorbent assay was employed to measure the levels of testosterone(T), luteinizing hormone(LH), follicle-stimulating hormone(FSH), cortisol(COR), gonadotropin-releasing hormone(GnRH), and estradiol(E_2) in the serum and the content of fructose in seminal plasma. Hematoxylin-eosin(HE) staining was employed to observe the pathological changes in the testicular tissue. The sperm quality was evaluated based on the density, viability, survival rate, and deformation rate of sperms. Western blot was employed to determine the expression of proteins involved in proliferation and apoptosis in the testicular tissue. The results showed that HJCT, HJSY, and HJCW significantly increased the grip strength, anal temperature, locomotor activities, weight-bearing swimming time, and sexual behaviors, elevated the serum levels of T, LH, GnRH, and E_2, lowered the levels of COR and FSH, and increased the wet weight of the testicular tissue in the model mice. In addition, they increased the density, viability, and survival and reduced the deformation rate of sperms, increased the content of fructose in the seminal plasma, alleviated the pathological changes and inhibited apoptosis in the testicular tissue in the model mice. HJCT was superior to HJSY and HJCW in ameliorating the hypothalamic-pituitary-gonadal axis(HPGA) disorder, enhancing sexual behaviors, and improving sperm quality. HJCW outperformed HJCT and HJSY in inhibiting apoptosis in the testicular tissue. In conclusion, HJCT, HJSY, and HJCW can improve the reproductive function and inhibit apoptosis in the testicular tissue of the male mice with kidney essence deficiency by ameliorating the HPGA disorder.
本研究旨在揭示黄精不同部位对因性交过度所致肾阴虚雄性小鼠生殖功能障碍的影响及其作用机制。选取50只性功能良好的雄性ICR小鼠，随机分为正常组（NC）、模型组（MC）、黄精正丁醇部位组（0.4 g·kg⁻¹，HJCT）、黄精其余部位组（0.4 g·kg⁻¹，HJSY）和黄精超微粉组（1.0 g·kg⁻¹，HJCW）。采用柯立芝效应诱导小鼠性交过度，建立肾阴虚生殖功能障碍小鼠模型。造模同时给予相应药物，连续给药8周。测量肛温、握力、自主活动、负重游泳时间和性行为（捕捉、跨骑和射精）。采用酶联免疫吸附测定法检测血清中睾酮（T）、黄体生成素（LH）、卵泡刺激素（FSH）、皮质醇（COR）、促性腺激素释放激素（GnRH）和雌二醇（E₂）水平以及精浆中果糖含量。采用苏木精-伊红（HE）染色观察睾丸组织病理变化。根据精子密度、活力、存活率和畸形率评估精子质量。采用蛋白质免疫印迹法检测睾丸组织中增殖和凋亡相关蛋白的表达。结果显示，HJCT、HJSY和HJCW显著提高模型小鼠的握力、肛温、自主活动、负重游泳时间和性行为，升高血清T、LH、GnRH和E₂水平，降低COR和FSH水平，增加睾丸组织湿重。此外，它们增加精子密度、活力和存活率，降低精子畸形率，增加精浆中果糖含量，减轻模型小鼠睾丸组织病理变化并抑制其凋亡。在改善下丘脑-垂体-性腺轴（HPGA）紊乱、增强性行为和提高精子质量方面，HJCT优于HJSY和HJCW。在抑制睾丸组织凋亡方面，HJCW优于HJCT和HJSY。综上所述，HJCT、HJSY和HJCW可通过改善HPGA紊乱提高肾阴虚雄性小鼠的生殖功能并抑制睾丸组织凋亡。
