Evans Sarah E, Valentine Meagan E, Gallimore Fallon, Meka Yogesh, Koehler Samuel I, Yu Hongwei D, Valentovic Monica A, Long Timothy E
Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, United States.
Department of Pharmaceutical Sciences, School of Pharmacy, Marshall University, Huntington, WV 25755, United States.
J Appl Microbiol. 2025 Jan 6;136(1). doi: 10.1093/jambio/lxae305.
Disulfiram (Antabuse®) is an oral alcohol sobriety medication that exhibits antimicrobial activity against Gram-positive facultative anaerobes. The aims of this study were to measure the antimicrobial activity against anaerobic bacteria of the gut human microbiome and establish the extent that disulfiram alters the microbial composition of the ileum, cecum, and feces using C57BL/6 mice.
Antimicrobial susceptibility testing by the microdilution method revealed that disulfiram inhibits the in vitro growth of gut anaerobic species of Bacteroides, Clostridium, Peptostreptococcus, and Porphyromonas. Differential sequencing of 16S rRNA isolated from the ileum, cecum, and feces contents of treated vs. untreated mice showed that disulfiram enriches the Gram-negative enteric population. In female mice, the enrichment was greatest in the ileum, whereas the feces composition in male mice was the most heavily altered.
Daily administration of oral disulfiram depletes the enteric Gram-positive anaerobe population as predicted by the minimum inhibitory concentration data for isolates from the human gut microbiota.
双硫仑（安塔布司®）是一种口服戒酒药物，对革兰氏阳性兼性厌氧菌具有抗菌活性。本研究的目的是测定其对人类肠道微生物群中厌氧菌的抗菌活性，并利用C57BL/6小鼠确定双硫仑改变回肠、盲肠和粪便微生物组成的程度。
通过微量稀释法进行的抗菌药敏试验表明，双硫仑可抑制肠道厌氧菌种拟杆菌属、梭菌属、消化链球菌属和卟啉单胞菌属的体外生长。对处理过的小鼠与未处理的小鼠的回肠、盲肠和粪便内容物中分离出的16S rRNA进行差异测序，结果显示双硫仑可使革兰氏阴性肠道菌群富集。在雌性小鼠中，回肠中的富集最为明显，而雄性小鼠粪便的组成变化最大。
如从人类肠道微生物群分离出的菌株的最低抑菌浓度数据所预测的那样，每日口服双硫仑会使肠道革兰氏阳性厌氧菌数量减少。
