Dong Wenke, Zhang Yujuan, Wang Yan, Zhao Chunxu
Key Laboratory of Grassland Ecosystem (Gansu Agricultural University), Ministry of Education, Lanzhou, 730070, China.
College of Grassland Science, Gansu Agricultural University, Lanzhou, 730070, China.
BMC Plant Biol. 2024 Dec 19;24(1):1195. doi: 10.1186/s12870-024-05914-8.
Poa pratensis is a predominant cool-season turfgrass utilized in urban landscaping and ecological management. It is extensively employed in turf construction and in the regulation of ecological environments. However, it is susceptible to powdery mildew, a prevalent disease in humid regions. Currently, the primary control measure for powdery mildew involves the application of pesticides, a practice that is both costly and environmentally detrimental. Developing superior disease-resistant cultivars represents a more cost-effective and sustainable strategy for managing turfgrass diseases. Furthermore, an in-depth investigation into the response mechanisms of P. pratensis to powdery mildew infection could significantly advance research on the identification of disease resistance genes and the molecular breeding of resistant varieties.
In this study, we first assessed the disease incidence across various disease-resistant P. pratensis cultivars and subsequently examined alterations in their in vivo redox states. We employed isobaric tags for relative and absolute quantification (iTRAQ) proteomics alongside non-targeted metabolomics to elucidate the response mechanisms of P. pratensis to powdery mildew invasion. A comprehensive analysis of the shared KEGG pathways among differentially abundant proteins (DAPs) and differentially enriched metabolites (DEMs) led to the identification of four common KEGG pathways. Notably, the phenylpropanoid biosynthesis pathway, enriched in both examined P. pratensis cultivars, was selected for further investigation. This analysis indicated that lignin biosynthesis plays a crucial role in the response of P. pratensis to powdery mildew infection.
The findings of this study enhance our understanding of the mechanisms underlying powdery mildew resistance in P. pratensis and serve as a valuable reference for the selection of powdery mildew-resistant cultivars, as well as for the identification and application of associated disease resistance genes.
Not applicable.
草地早熟禾是城市绿化和生态管理中主要使用的冷季型草坪草。它广泛应用于草坪建设和生态环境调节。然而，它易受白粉病影响，白粉病是潮湿地区的一种常见病害。目前，白粉病的主要防治措施是使用农药，这种做法既昂贵又对环境有害。培育优良的抗病品种是管理草坪草病害更具成本效益和可持续性的策略。此外，深入研究草地早熟禾对白粉病感染的反应机制，可显著推进抗病基因鉴定和抗性品种分子育种的研究。
在本研究中，我们首先评估了各种抗病草地早熟禾品种的发病率，随后检测了它们体内氧化还原状态的变化。我们采用相对和绝对定量同位素标记（iTRAQ）蛋白质组学以及非靶向代谢组学来阐明草地早熟禾对白粉病入侵的反应机制。对差异丰富蛋白质（DAPs）和差异富集代谢物（DEMs）之间共享的KEGG途径进行综合分析，确定了四条常见的KEGG途径。值得注意的是，在所检测的两个草地早熟禾品种中均富集的苯丙烷生物合成途径被选作进一步研究对象。该分析表明木质素生物合成在草地早熟禾对白粉病感染的反应中起关键作用。
本研究结果增进了我们对草地早熟禾抗白粉病机制的理解，为白粉病抗性品种的选择以及相关抗病基因的鉴定和应用提供了有价值的参考。
不适用。
