Li Lekang, Liang Xuanguang, Zhang Jiatong, Xu Qun, Wang Li, Gao Xiaoping, Song Xiangchun, Zhang Bao, Huang Dan, Wang Hong, Wang Xianyong, Luo Zhen, Kong Chiping, Lu Jianguo
Jiujiang Academy of Agricultural Sciences, Jiujiang, 332000, Jiangxi, China.
School of Marine Sciences, Sun Yat-sen University, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519082, Guangdong, China.
Sci Data. 2024 Dec 19;11(1):1399. doi: 10.1038/s41597-024-04223-x.
Ochetobius elongatus, a critically endangered species found in the Yangtze River was the subject of our study in which we leveraged PacBio and Hi-C data to assemble a chromosome-scale genome. This assembly comprises 24 pseudo-chromosomes, yielding a genome size of 883.1 Mb with a scaffold N50 length of 35.1 Mb, indicative of a highly contiguous assembly. A BUSCO assessment ascertained the comprehensiveness of the genome at 98.3%. Annotation efforts identified 28,674 putative protein-coding genes, with 44.63% of the assembled genome annotated as repetitive sequences. Collinearity analysis between O. elongatus and two other species from the family Xenocyprididae revealed high collinearity, indicating good assembly quality of O. elongatus The completion of the O. elongatus genome assembly in this study signifies a critical advancement for its conservation, enabling deeper insights into its genetic diversity and facilitating the development of targeted preservation strategies.
长鳍鲌是一种在长江中发现的极度濒危物种，是我们研究的对象。在这项研究中，我们利用PacBio和Hi-C数据组装了一个染色体水平的基因组。该组装包含24条假染色体，基因组大小为883.1 Mb，支架N50长度为35.1 Mb，表明组装高度连续。BUSCO评估确定基因组的完整性为98.3%。注释工作鉴定出28,674个推定的蛋白质编码基因，组装基因组的44.63%被注释为重复序列。长鳍鲌与鲤科其他两个物种之间的共线性分析显示出高度共线性，表明长鳍鲌的组装质量良好。本研究中长鳍鲌基因组组装的完成标志着其保护工作取得了关键进展，能够更深入地了解其遗传多样性，并有助于制定有针对性的保护策略。
