Rumiani Mohammad, Zouhar Miloslav, Karegar Akbar, Hamzehzarghani Habiballah, Tahmasebi Ahmad, Rashidifard Milad
Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran.
Department of Plant Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic.
J Nematol. 2024 Dec 15;56(1):20240047. doi: 10.2478/jofnem-2024-0047. eCollection 2024 Mar.
Molecular data should be combined with morphological data to enhance the reliability of phylogenetic and diagnostic studies on nematodes. In this study, the citrus nematode collected from citrus orchards in different localities in Fars province, southern Iran, was characterized using the partial sequencing of ITS rDNA, D2-D3 of 28S rDNA and COI mtDNA genes. We also morphometrically characterized the second-stage juveniles (J2) and male specimens. The results showed that is a genetically homogeneous species, and only minor nucleotide differences were detected among the populations. Phylogenetic studies demonstrated that most Iranian populations were grouped together, and there were no differences among the populations. However, sequence alignment of ITS, D2-D3 of 28S rDNA and COI mtDNA revealed 17, 24, and 16 single nucleotide variations (SNVs) and 11, 12, and 11 single-nucleotide polymorphisms (SNPs), respectively. The results of the morphometric analysis showed slight morphometric differences among and within the populations of . The morphometric differences among citrus nematode populations and the haplotype topology of the populations did not correlate with their geographical origin and host type. The constructed phylogenetic trees showed a close relationship between and . In addition, the phylogenetic relationships showed that is the closest taxon to The results of this study provide a better understanding of the diversity of populations and may shed light on the genetic variation of citrus nematode.
分子数据应与形态学数据相结合，以提高线虫系统发育和诊断研究的可靠性。在本研究中，对从伊朗南部法尔斯省不同地区的柑橘果园采集的柑橘线虫，使用ITS rDNA、28S rDNA的D2-D3和COI mtDNA基因的部分测序进行了特征分析。我们还对第二阶段幼虫（J2）和雄性标本进行了形态测量特征分析。结果表明，该线虫是一个基因同质的物种，在种群之间仅检测到微小的核苷酸差异。系统发育研究表明，大多数伊朗种群聚集在一起，种群之间没有差异。然而，ITS、28S rDNA的D2-D3和COI mtDNA的序列比对分别揭示了17、24和16个单核苷酸变异（SNV）以及11、12和11个单核苷酸多态性（SNP）。形态测量分析结果表明，该线虫种群之间和种群内部存在轻微的形态测量差异。柑橘线虫种群之间的形态测量差异和种群的单倍型拓扑结构与其地理起源和寄主类型无关。构建的系统发育树显示该线虫与另一线虫之间关系密切。此外，系统发育关系表明，该线虫是与另一线虫最接近的分类单元。本研究结果有助于更好地理解该线虫种群的多样性，并可能揭示柑橘线虫的遗传变异。
