Wang Ying, Li Zichao, Liu Yaoguang, Ding Yu, Wang Zhongzhi
Department of Dermatology, Shanghai Fourth People's Hospital, Tongji University School of Medicine, Shanghai, China.
Department of Burn and Plastic Surgery, 905 Hospital of the People's Liberation Army Navy, Shanghai, China.
J Int Med Res. 2024 Dec;52(12):3000605241286992. doi: 10.1177/03000605241286992.
This study aims to inform the prevention and treatment of infections in China by analyzing the molecular epidemiology and distribution patterns of the pathogen.
A cross-sectional study was conducted involving a retrospective analysis of 64 clinical strains of isolated from January 2018 to April 2023 across various domestic regions. Patient information was collected from human immunodeficiency virus-positive individuals in multiple hospitals located in 19 cities within China's southeastern coastal and southwestern regions. A VITEK MS mass spectrometer was employed for strain identification. Variants and mating types were amplified using polymerase chain reaction (PCR), while PCR multilocus sequence typing (MLST) was utilized for molecular genetic analysis of the strains. Evolutionary trees were constructed using MEGA software.
The study identified a male predominance (2.37:1) among the 64 strains, with a median patient age of 38.5 years. Most strains (79.69%) were isolated from cerebrospinal fluid. All strains belonged to the Aα, VN I type, with MLST revealing three sequence types: ST5 (89.06%), ST32 (7.81%), and ST186 (3.13%).
From 2018 to 2023, infections in China were predominantly found in middle-aged men, and the VN I genotype was the most common among the isolated strains.
本研究旨在通过分析病原体的分子流行病学和分布模式，为中国感染病的预防和治疗提供信息。
开展一项横断面研究，对2018年1月至2023年4月期间从国内不同地区分离出的64株临床菌株进行回顾性分析。从中国东南沿海和西南地区19个城市的多家医院的人类免疫缺陷病毒阳性个体中收集患者信息。采用VITEK MS质谱仪进行菌株鉴定。使用聚合酶链反应（PCR）扩增变体和交配型，同时利用PCR多位点序列分型（MLST）对菌株进行分子遗传学分析。使用MEGA软件构建进化树。
该研究在64株菌株中发现男性占主导（2.37:1），患者中位年龄为38.5岁。大多数菌株（79.69%）从脑脊液中分离得到。所有菌株均属于Aα、VN I型，MLST显示三种序列类型：ST5（89.06%）、ST32（7.81%）和ST186（3.13%）。
2018年至2023年期间，中国的感染病主要见于中年男性，在分离出的菌株中VN I基因型最为常见。
