Li Na, Zhang Yan, Zhang Dongyu, Zhang Xiaoli, Wei Ning, Liu Xiuhui
Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China.
Lanzhou University First Hospital, Lanzhou, 730000, China.
Anal Methods. 2025 Jan 23;17(4):783-791. doi: 10.1039/d4ay01766h.
It is widely known that homocysteine (Hcy) is associated with the pathogenesis of many clinical diseases, and its quantitative detection can help early treatment of related diseases. The selection of materials with excellent properties is important for the construction of electrochemical sensing platforms and quantitative detection of Hcy. In this study, two nanocomposites, Au nanoparticles/hollow carbon spheres (AuNPs@HCS) and Au nanoparticles/solid carbon spheres (AuNPs@SCS), were prepared first. Then their morphology, structure and electrochemical behavior were investigated. And the comparison found that the HCSs have a large electroactive surface area and outstanding conductivity. Thanks to the catalytic properties of AuNPs and the synergistic effect of HCSs, AuNPs@HCS/GCE showed excellent electrochemical sensing performance for a wide range of Hcy concentrations with a detection limit down to 4.44 nM (S/N = 3). Moreover, the electrochemical mechanism of Hcy on Au@HCS/GCE is discussed in detail. Finally, in the actual serum sample assay, the sensor is used to detect Hcy directly and the recovery rate is satisfactory. This work may provide a valid method for the quantitative determination of Hcy in the future.
众所周知，同型半胱氨酸（Hcy）与许多临床疾病的发病机制相关，其定量检测有助于相关疾病的早期治疗。选择具有优异性能的材料对于构建电化学传感平台和Hcy的定量检测至关重要。在本研究中，首先制备了两种纳米复合材料，即金纳米颗粒/空心碳球（AuNPs@HCS）和金纳米颗粒/实心碳球（AuNPs@SCS）。然后研究了它们的形态、结构和电化学行为。比较发现，空心碳球具有较大的电活性表面积和出色的导电性。由于金纳米颗粒的催化性能和空心碳球的协同作用，AuNPs@HCS/GCE对广泛浓度的Hcy表现出优异的电化学传感性能，检测限低至4.44 nM（S/N = 3）。此外，详细讨论了Hcy在Au@HCS/GCE上的电化学机理。最后，在实际血清样本检测中，该传感器用于直接检测Hcy，回收率令人满意。这项工作可能为未来Hcy的定量测定提供一种有效的方法。
