Zhao Ziqiao, Ke Junhong, Liu Mengfan, Chen Zuoxin, Li Shuwen, Wei Wuque, Mei Kun, Huang Shujian
Waterfowl Infectious Disease Diagnosis and Prevention Research Team, School of Animal Science and Technology, Foshan University, Foshan, People's Republic of China.
Avian Pathol. 2025 Apr;54(2):175-184. doi: 10.1080/03079457.2024.2373365. Epub 2024 Dec 20.
The Duck Tembusu virus (DTMUV) was first reported in China in 2010 and has since caused substantial economic losses in the poultry breeding industry. In the autumn of 2022, an outbreak of an infectious disease resembling DTMUV was reported in Guangdong Province, China, which caused significantly high mortality in goose embryos, and decreased egg production. This study identified one strain of the new subgenotype 3 of DTMUV, designated as DTMUV GDZQ2022, responsible for these effects. Comprehensive genomic sequencing of this strain was conducted to analyse its genetic variations. Additionally, the isolated and purified virus was inoculated into goose embryos and goslings to assess its pathogenicity. The GDZQ2022 genome displayed over 88% nucleotide homology with other DTMUV strains from China and Southeast Asia. Phylogenetic analysis of the E gene classified GDZQ2022 within the subgenotype 3 of DTMUV. Pathogenicity experiments on goose embryos and goslings showed that the GDZQ2022 strain induced typical clinical signs of DTMUV, particularly severe neurological manifestations. Although GDZQ2022 exhibited high virulence in goose embryos, its virulence in goslings was minimal, resulting in a low mortality rate. Pathological examinations detected significant histological lesions in the brains, livers, and spleens of the infected goslings. In conclusion, this study presents the first evidence of a novel DTMUV strain proliferating among young geese in China, underscoring the genetic diversity of DTMUV and contributing to our understanding of the pathogenicity of the subgenotype 3 Tembusu virus in goose embryos and goslings.Pathogenicity of subgenotype 3 duck Tembusu virus in goose embryos and goslings was shown for the first time.DTMUV GDZQ2022 strain is highly pathogenic in goose embryos.The virulence of the DTMUV GDZQ2022 strain in goslings is relatively mild.Infected goslings exhibit typical clinical manifestations, with a low mortality rate.
鸭坦布苏病毒（DTMUV）于2010年首次在中国被报道，自那时起已在家禽养殖业中造成了巨大的经济损失。2022年秋季，中国广东省报告了一起类似DTMUV的传染病疫情，该疫情导致鹅胚死亡率显著升高，产蛋量下降。本研究鉴定出一株DTMUV新的亚基因型3毒株，命名为DTMUV GDZQ2022，它是造成这些影响的原因。对该毒株进行了全面的基因组测序以分析其遗传变异。此外，将分离纯化的病毒接种到鹅胚和雏鹅中以评估其致病性。GDZQ2022基因组与来自中国和东南亚的其他DTMUV毒株显示出超过88%的核苷酸同源性。对E基因的系统发育分析将GDZQ2022归类于DTMUV的亚基因型3。对鹅胚和雏鹅的致病性实验表明，GDZQ2022毒株引发了DTMUV的典型临床症状，特别是严重的神经症状。尽管GDZQ2022在鹅胚中表现出高毒力，但其在雏鹅中的毒力极小，导致死亡率较低。病理检查在受感染雏鹅的脑、肝和脾中检测到明显的组织学病变。总之，本研究首次提供了一种新型DTMUV毒株在中国雏鹅中增殖的证据，强调了DTMUV的遗传多样性，并有助于我们了解亚基因型3坦布苏病毒在鹅胚和雏鹅中的致病性。首次展示了亚基因型3鸭坦布苏病毒在鹅胚和雏鹅中的致病性。DTMUV GDZQ2022毒株对鹅胚具有高致病性。DTMUV GDZQ2022毒株在雏鹅中的毒力相对较弱。受感染的雏鹅表现出典型的临床表现，死亡率较低。
