Mousavi Seyyed Mojtaba, Chiang Wei-Hung, Gholami Ahmad
Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City 106335, Taiwan.
Sustainable Electrochemical Energy Development (SEED) Center, National Taiwan University of Science and Technology, Taipei City 10607, Taiwan.
J Mater Chem B. 2025 Jan 15;13(3):965-984. doi: 10.1039/d4tb01303d.
The increasing effective, detectable, and targeted anticancer systems are driven by the growing cancer incidence and the side effects of current drugs. Natural products like saponin and apigenin have emerged as valuable compounds for precise treatment. Recent advancements in bioactive metal-organic frameworks (MOFs) have introduced multifunctional particles suitable for cellular imaging, targeted drug delivery, and early cancer treatment. In this study, bioactive ZIF-67 and ZIF-8 materials were synthesized, incorporating zinc nitrate and natural bioactive compounds such as saponin and apigenin to sensitize and deliver the material to damaged cancer tissue. The characterization of these bioactive nanostructures involved FT-IR, TEM, EDX, FESEM, and BET analysis. The study quantified the loading and release of natural products within the ZIF structure. Cytotoxicity assessments of drug-loaded MOFs were conducted on human oral cavity carcinoma cell lines OSCC, Hep-G2, Raji, MCF-7, and PDL under conditions. Flow cytometry analysis identified the combination of bioactive ZIF-67 and saponins as the most effective in inducing apoptosis. Finally, a novel synthesis of bioactive MOF compounds was developed with dual applications: drug delivery and cancer imaging, featuring a unique attribute that minimizes side effects on normal cells.
日益有效的、可检测的和靶向性抗癌系统是由不断上升的癌症发病率以及现有药物的副作用所驱动的。皂苷和芹菜素等天然产物已成为精确治疗的有价值化合物。生物活性金属有机框架（MOF）的最新进展引入了适用于细胞成像、靶向药物递送和早期癌症治疗的多功能颗粒。在本研究中，合成了生物活性ZIF-67和ZIF-8材料，将硝酸锌与皂苷和芹菜素等天然生物活性化合物结合，以使材料对受损癌组织产生敏化作用并进行递送。这些生物活性纳米结构的表征涉及傅里叶变换红外光谱（FT-IR）、透射电子显微镜（TEM）、能量色散X射线光谱（EDX）、场发射扫描电子显微镜（FESEM）和比表面积分析（BET）。该研究对ZIF结构内天然产物的负载和释放进行了量化。在特定条件下，对载药MOF进行了对人口腔癌细胞系OSCC、Hep-G2、Raji、MCF-7和PDL的细胞毒性评估。流式细胞术分析确定生物活性ZIF-67和皂苷的组合在诱导细胞凋亡方面最为有效。最后，开发了一种具有双重应用的生物活性MOF化合物的新型合成方法：药物递送和癌症成像，其具有将对正常细胞的副作用降至最低的独特特性。
