Sinha Aishi, Taneja Sonali, Shetty Devi Charan, Bhalla Vidhi Kiran
Department of Conservative Dentistry and Endodontics, I.T.S Centre for Dental Studies and Research, Ghaziabad, India.
Department of Oral Pathology, I.T.S Centre for Dental Studies and Research, Ghaziabad, India.
Eur Endod J. 2024 Dec;9(4):411-417. doi: 10.14744/eej.2024.75983.
This study aimed to evaluate the antibacterial efficacy of different concentrations of GH12 on a simulated multispecies biofilm comprising Enterococcus faecalis, Streptococcus mutans, Fusobacterium nucleatum and Porphyromonas gingivalis.
Single rooted teeth were decoronated, cut into 1.5 mm sections to obtain dentine discs which were randomly allocated into five groups: (n=12 each), Group 1: Phosphate Buffered Solution (PBS) - negative control, Group II: 5% Sodium hypochlorite (NaOCl) - positive control, Group III: Minimum Inhibitory Concentration (MIC) of GH12, Group IV: 2x MIC of GH12, Group V: 4x MIC of GH12. Colony forming units, Crystal violet assay and scanning electron microscopy examinations were performed. One-way ANOVA and Turkey's test were applied for statistical analysis using the SPSS software version 22.0.
Group II (NaOCl) showed maximum reduction in bacterial load followed by Group V (GH12 16mg/mL) with no statistically significant difference (p=1.000). On comparing the mean CFU reduction, the maximum reduction was identified for S. mutans and the least was for P. gingivalis. There was marked erosion observed in the NaOCl group whereas the GH12 group showed no erosive changes in the morphology and no bacterial colonies was identified.
The findings revealed that GH12 at higher concentrations inhibits and disrupts the growth of multispecies endodontic biofilm comparable to NaOCl but without erosive effects to the dentine, further highlighting its potential to be used as an antimicrobial solution. (EEJ-2023-10-147).
本研究旨在评估不同浓度的GH12对包含粪肠球菌、变形链球菌、具核梭杆菌和牙龈卟啉单胞菌的模拟多菌种生物膜的抗菌效果。
将单根牙去冠，切成1.5毫米的切片以获得牙本质盘，将其随机分为五组（每组n = 12）：第1组：磷酸盐缓冲溶液（PBS）——阴性对照；第II组：5%次氯酸钠（NaOCl）——阳性对照；第III组：GH12的最低抑菌浓度（MIC）；第IV组：2倍MIC的GH12；第V组：4倍MIC的GH12。进行菌落形成单位、结晶紫测定和扫描电子显微镜检查。使用SPSS 22.0软件进行单因素方差分析和土耳其检验以进行统计分析。
第II组（NaOCl）的细菌载量降低最多，其次是第V组（GH12 16mg/mL），两者无统计学显著差异（p = 1.000）。比较平均CFU降低情况，变形链球菌的降低最多，牙龈卟啉单胞菌的降低最少。在NaOCl组中观察到明显的侵蚀，而GH12组在形态上未显示侵蚀变化，也未发现细菌菌落。
研究结果表明，高浓度的GH12可抑制并破坏多菌种牙髓生物膜的生长，其效果与NaOCl相当，但对牙本质无侵蚀作用，进一步凸显了其作为抗菌溶液的潜力。（EEJ - 2023 - 10 - 147）
