Wu Hong, Fujioka Yoshihiko, Iwai Noritaka, Sakaguchi Shoichi, Suzuki Youichi, Nakano Takashi
Project Team for Study of Nanotransportation System, Center for Medical Research and Development, Osaka Medical and Pharmaceutical University, 2-7 Daigaku-Machi, Takatsuki, Osaka, 569-8686, Japan.
Department of Microbiology and Infection Control, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigaku-Machi, Takatsuki, Osaka, 569-8686, Japan.
Med Mol Morphol. 2025 Jun;58(2):126-136. doi: 10.1007/s00795-024-00416-w. Epub 2024 Dec 20.
Helicobacter pylori possesses an intrabacterial nanotransportation system (ibNoTS) for transporting VacA, CagA, and urease within the bacterial cytoplasm. This system is controlled by the extrabacterial environment. The transport routes of the system for VacA have not yet been studied in detail. In this study, we demonstrated by immunoelectron microscopy that VacA localizes closely with the MreB filament in the bacterium, and the MreB polymerization inhibitor A22 obstructs the transport of VacA by ibNoTS. These findings indicate that the route of ibNoTS for VacA is closely associated with the MreB filament Additionally, it was confirmed that VacA does not closely associate with the bacterial filament FtsZ, which is involved in the transport of the virulence factor urease, as previously suggested. We propose that the route of ibNoTS for VacA is associated with the MreB filament in H. pylori.
幽门螺杆菌拥有一种细菌内纳米运输系统（ibNoTS），用于在细菌细胞质内运输空泡毒素A（VacA）、细胞毒素相关蛋白A（CagA）和脲酶。该系统受细菌外环境控制。VacA的该系统运输途径尚未得到详细研究。在本研究中，我们通过免疫电子显微镜证明，VacA在细菌内与MreB丝紧密定位，并且MreB聚合抑制剂A22阻碍ibNoTS对VacA的运输。这些发现表明，ibNoTS对VacA的运输途径与MreB丝密切相关。此外，正如之前所提出的，已证实VacA与参与毒力因子脲酶运输的细菌丝FtsZ没有紧密关联。我们提出，ibNoTS对VacA的运输途径与幽门螺杆菌中的MreB丝相关。
