Byrne Audrey, Diener Christian, Brown Bryan P, Maust Brandon S, Feng Colin, Alinde Berenice L, Gibbons Sean M, Koch Meghan, Gray Clive M, Jaspan Heather B, Nyangahu Donald D
Seattle Children's Research Institute, Seattle, WA, USA.
Institute For Systems Biology, Seattle, WA, 98109, USA.
Microbiome. 2024 Dec 20;12(1):261. doi: 10.1186/s40168-024-01973-z.
Infants exposed to HIV but uninfected have altered immune profiles which include heightened systemic inflammation. The mechanism(s) underlying this phenomenon is unknown. Here, we investigated differences in neonatal gut bacterial and viral microbiome and associations with inflammatory biomarkers in plasma. Further, we tested whether HIV exposure impacts antibody-microbiota binding in neonatal gut and whether antibodies in breast milk impact the growth of commensal bacteria.
Neonates exposed to HIV but uninfected (nHEU) exhibited altered gut bacteriome and virome compared to unexposed neonates (nHU). In addition, HIV exposure differentially impacted IgA-microbiota binding in neonates. The relative abundance of Blautia spp. in the whole stool or IgA-bound microbiota was positively associated with plasma concentrations of C-reactive protein. Finally, IgA from the breast milk of mothers living with HIV displayed a significantly lower ability to inhibit the growth of Blautia coccoides which was associated with inflammation in nHEU.
nHEU exhibits profound alterations in gut bacterial microbiota with a mild impact on the enteric DNA virome. Elevated inflammation in nHEU could be due to a lower capacity of breast milk IgA from mothers living with HIV to limit growth the of gut bacteria associated with inflammation. Video Abstract.
暴露于HIV但未感染的婴儿具有改变的免疫特征，其中包括全身性炎症加剧。这一现象背后的机制尚不清楚。在此，我们研究了新生儿肠道细菌和病毒微生物群的差异以及与血浆中炎症生物标志物的关联。此外，我们测试了HIV暴露是否会影响新生儿肠道中抗体与微生物群的结合，以及母乳中的抗体是否会影响共生细菌的生长。
与未暴露的新生儿（nHU）相比，暴露于HIV但未感染的新生儿（nHEU）表现出肠道细菌群落和病毒群落的改变。此外，HIV暴露对新生儿IgA与微生物群的结合有不同影响。全粪便或IgA结合微生物群中Blautia属的相对丰度与血浆C反应蛋白浓度呈正相关。最后，感染HIV母亲母乳中的IgA抑制球形布劳特氏菌生长的能力显著降低，这与nHEU中的炎症有关。
nHEU在肠道细菌微生物群中表现出深刻变化，对肠道DNA病毒群落影响较小。nHEU中炎症升高可能是由于感染HIV母亲母乳中的IgA限制与炎症相关肠道细菌生长的能力较低。视频摘要。
