Sun Tao, Sun Mei-Li, Lin Lu, Gao Jian, Wang Kaifeng, Ji Xiao-Jun
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
School of Marine and Bioengineering, Yancheng Institute of Technology, No. 211 Jianjun Road, Yancheng 224051, People's Republic of China.
J Agric Food Chem. 2025 Jan 8;73(1):100-109. doi: 10.1021/acs.jafc.4c09990. Epub 2024 Dec 21.
Succinic acid is an essential bulk chemical with wide-ranging applications in materials, food, and pharmaceuticals. With the advancement of biotechnology, there has been a surge in focus on low-carbon sustainable microbial synthesis methods for producing biobased succinic acid. Due to its high intrinsic acid tolerance, has gained recognition as a competitive chassis for the industrial manufacture of succinic acid. This review summarizes the research progress on succinic acid biomanufacturing using . First, it introduces the major metabolic routes for succinic acid biosynthesis and the pertinent engineering approaches for building efficient cell factories. Subsequently, we offer a review of methods employed for succinic acid synthesis by utilizing alternative substrates as well as the relevant optimization strategies for the fermentation process. Finally, future research directions for improving succinic acid biomanufacturing in are delineated in light of the recent progress, obstacles, and trends in this area.
琥珀酸是一种重要的大宗化学品，在材料、食品和制药等领域有着广泛应用。随着生物技术的发展，人们越来越关注用于生产生物基琥珀酸的低碳可持续微生物合成方法。由于其具有较高的内在耐酸性，已成为工业生产琥珀酸的有竞争力的底盘细胞。本综述总结了利用[底盘细胞名称未给出]进行琥珀酸生物制造的研究进展。首先，介绍了琥珀酸生物合成的主要代谢途径以及构建高效细胞工厂的相关工程方法。随后，我们综述了利用[底盘细胞名称未给出]以替代底物合成琥珀酸所采用的方法以及发酵过程的相关优化策略。最后，根据该领域的最新进展、障碍和趋势，阐述了未来在[底盘细胞名称未给出]中改进琥珀酸生物制造的研究方向。
