Lv Yuxia, Dong Liansheng, Wang Xiatong, Shen Linhong, Lu Wenbo, Si Fan, Zhao Yaoyao, Zhu Guanju, Ding Yiting, Cao Shujun, Cao Jiajia, Lu Jie, Ma Chuanxi, Chang Cheng, Zhang Haiping
College of Agronomy, Anhui Agricultural University, Key Laboratory of Wheat Biology and Genetic Improvement On Southern Yellow & Huai River Valley, Ministry of Agriculture, Hefei, 230036, Anhui, China.
BMC Plant Biol. 2024 Dec 21;24(1):1228. doi: 10.1186/s12870-024-05956-y.
Genetic dissection of yield-related traits can be used to improve wheat yield through molecular design breeding. In this study, we genotyped 245 wheat varieties and measured 13 yield-related plant height-, grain-, and spike-related traits, in seven environments, and identified 778 loci for these traits by genome-wide association study (GWAS) using single- and multi-locus models. Among these loci, nine were major, of which seven were novel, including Qph/lph.ahau-7A for plant height (PH) and leaf pillow height (LPH), Qngps/sps.ahau-1A for number of grains per spike (NGPS) and spikelet number per spike (SPS), Qsd.ahau-2B.1 and Qsd.ahau-5A.2 for spikelet density (SD), Qlph.ahau-7B.2 for LPH, Qgl.ahau-7B.3 for grain length (GL), and Qsl.ahau-3A.3 for spike length (SL). Through marker development, re-GWAS, gene annotation and cloning, and sequence variation, haplotype, and expression analyses, we confirmed two novel major loci and identified potential candidate genes, TraesCS7A02G118000 (named TaF-box-7A) and TraesCS1A02G190200 (named TaBSK2-1A) underlying Qph/lph.ahau-7A for PH-related traits and Qngps/sps.ahau-1A for spike-related traits. We also reported two favorable haplotypes, including TaF-box-Hap1 associated with low PH and LPH and TaBSK2-Hap3 associated with high NGPS and SPS. In summary, these findings can be applied to improve wheat yield and enrich our understanding of the complex genetic mechanisms of yield-related traits.
产量相关性状的遗传解析可用于通过分子设计育种提高小麦产量。在本研究中，我们对245个小麦品种进行了基因分型，并在7种环境下测量了13个与产量相关的株高、籽粒和穗部相关性状，通过全基因组关联研究（GWAS）使用单基因座和多基因座模型为这些性状鉴定了778个基因座。在这些基因座中，9个是主效基因座，其中7个是新发现的，包括控制株高（PH）和叶枕高度（LPH）的Qph/lph.ahau-7A、控制每穗粒数（NGPS）和每穗小穗数（SPS）的Qngps/sps.ahau-1A、控制小穗密度（SD）的Qsd.ahau-2B.1和Qsd.ahau-5A.2、控制LPH的Qlph.ahau-7B.2、控制粒长（GL）的Qgl.ahau-7B.3和控制穗长（SL）的Qsl.ahau-3A.3。通过标记开发、重新GWAS、基因注释和克隆以及序列变异、单倍型和表达分析，我们确认了两个新的主效基因座，并鉴定了潜在的候选基因，即与PH相关性状的Qph/lph.ahau-7A的TraesCS7A02G118000（命名为TaF-box-7A）和与穗部相关性状的Qngps/sps.ahau-!A的TraesCS1A02G190200（命名为TaBSK2-1A）。我们还报道了两种有利的单倍型，包括与低PH和LPH相关的TaF-box-Hap1以及与高NGPS和SPS相关的TaBSK2-Hap3。总之，这些发现可用于提高小麦产量，并丰富我们对产量相关性状复杂遗传机制的理解。
